Presentation on theme: "William Kim and Nicole Kiess"— Presentation transcript:
1 William Kim and Nicole Kiess The Effect of Fruit Polyphenols on the Longevity of Caenorhabditis elegansWilliam Kim and Nicole Kiess
2 AbstractThe Effect of Fruit Polyphenols on the Longevity of C. elegansSarcopenia is defined as the loss of muscle mass, strength, and function associated with aging. As aging takes place, there is a sharp decrease in muscle mass and strength seen in both physically active individuals and physically inactive individuals. Sarcopenia is a major feature of aging in both humans and C. elegans, a model organism used for studying aging and longevity. Blueberry polyphenols have been found to increase the lifespan of C. elegans, seen in the pharynx contraction rate in adult C. elegans. C. elegans pumping rate of the pharynx is an indication of the age for the pumping rate of the pharynx decreases with age. Using various concentrations of different fruit polyphenols the lifespan of C. elegans will be measured by observing the pharynx contraction rate. Seventy-five worms will be fed two different concentrations of plum polyphenols, fifty C. elegans will be fed three different concentrations of strawberry polyphenols, and there will be one-hundred fifty worms in the control group. They all will be fed an equal amount of each fruit polyphenol. The average pumping rate of the pharynx per minute will be measured with the use of a Motic camera attached to a Zeiss Axiovert 40 CFL microscope.
4 Caenorhabditis elegans Image 1: C. elegans external appearanceImage 2: C. elegans internal appearance
5 C. Elegans Life CycleImage 3: C. elegans Life Cycle
6 Pharynx of C. elegansImage 4: Pharyngeal Structure of Caenorhabditis elegans
7 Literature ReviewWilson et. al. (2006) found that blueberry polyphenols were successful in increasing the lifespan and thermotolerance in C. elegansJames JosephFig. 2 Blueberry polyphenols extend lifespan and slow aging in C. elegansWilson et. al. 2006
8 Literature ReviewFigure 3: Blueberry polyphenols extend lifespan and slow aging in C. elegans
9 Literature ReviewFigure 4: Structural and functional declines in C. elegans pharynx from days 2-10.Chow et. al. 2006
10 Literature ReviewFigure 5: Visible Structural Decline in C. elegans as a result of SarcopeniaHerndon et. al. (2002)
11 PurposeTherefore the purpose of this experiment is to test the ability of different concentrations of fruit polyphenols on the prolonging of lifespan in C. elegans.HypothesisH(0): The pelargonidin chloride will show no effect on the prolonging of lifespanin C. elegans.H(0): The cyanidin chloride will show no effect on the prolonging of lifespan in C. elegansH(a): The pelargonidin chloride will show a positive increase in the lifespan of C. elegans.H(a): The cyanidin chloride will show a positive increase in the lifespan of C. elegans.
12 Pelgargonidin Choride MethodologyCaenorhabditis elegansControln = 150Pelargonidin Chloride 200 ug/mln = 50MeasurementAging of C. elegans measurement possible with the use of FUDR.Statistical AnalysisANOVA run by SPSS followed by a Scheffe Post Hoc TestPelargonidin Chloride 100ug/mlPelargonidin Chloride 50ug/mlCyanidin Chloride 200ug/mgn = 75Cyanidin Chloride50ug/mlN= 75Pelgargonidin ChorideN = 150Cyanidin ChorideN = 150
13 Protocols for Measurement Fruit polyphenol extracts is added to NGM agar plates of C. elegans on the first day of the lifespan assayLifespan scoring is initiated after hermaphrodites complete their final larval molt, on the first day of adulthoodPharynx pumping rates are measured on days 4, 6, 8, and 10 of C. elegans adulthood
14 Protocols for Measurement Pharynx contraction rate recorded with Motic Plus camera attached to a stereomicroscopePumps per minute measured
15 DoabilityMotic Plus with Stereomicroscope available in lab for pharynx contraction observationFruit polyphenols are easily obtainableC. elegans are easily obtainable and culturable
17 Bibliography“Aging Statistics.” Administration on Aging. (2009) 24 March <http://www.aoa.gov/AoARoot/Aging_Statistics/index.aspx> “Antioxidants.” McGraw-Hill Yearbook of Science & Technology. McGraw-Hill, “Antioxidants.” MedlinePlus. (2009) 9 Feb <http://www.nlm.nih.gov/medlineplus/antioxidants.html#cat1> Carlson, Rick E., Kirby, Brett S., Voyles, Wyatt F., and Dinenno, Frank A. “Evidence for impaired skeletal muscle contraction-induced rapid vasodilation in aging humans.” American Journal of Physiology – Heart and Circulatory Physiology 294 (2008): H1963-H1970. Joseph, James. "ARS Project: The effects of walnuts on age related changes in neuroprotection, inflammatory signaling and behavior (414597)." <http://www.ars.usda.gov/research/projects/projects.htm?ACCN_NO=414597>. Kenney, Richard A. Physiology of Aging: A Synopsis. Chicago, London. Year Book Medical Publishers, INC, Larsson, L., Grimby, G., and Karlsson, J.. “Muscle Strength and Speed of Movement in Relation to Age and Muscle Morphology.” Journal of Applied Physiology 46 (1979): "NCLD - Dyspraxia." NCLD - Home. 04 May 2009 <http://www.ncld.org/index.php?option=content&task=view&id=466>. Oliver, Rush H., Albury, Acchia N.J., and Mousseau, Timothy A.. “Programmed Cell Death in Flight Muscle Histolysis of the House Cricket.” Journal of Insect Physiology 53 (2007): Scalbert, Augustin, Johnson, Ian T., and Saltmarsh, Mike. “Polyphenols: antioxidants and beyond.” The American Journal of Clinical Nutrition 81 (2005): 215S-7S. “Types of contractions.” Muscle Physiology. (2006). University of California. 31 May < USDA/Agricultural Research Service. "Adding Walnuts To Good Diet May Help Older People Improve Motor And Behavioral Skills." ScienceDaily 25 April May 2009 <http://www.sciencedaily.com /releases/2009/04/ htm>. “What Is an Antioxidant?.” American Dietetic Association. (2006). 14 Sept <http:/www.eatright.org/cps/rde/xchg/ada/hs.xsl/home_9660_ENU_HTML.htm>. Wilson, Mark A., Shukitt-Hale, Barbara, Kalt, Wilhemina, Ingram, Donald K., Joseph, James A., and Wolkow, Catherine A. “Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans.” Aging Cell. 5 (2006):