1. Sequencing Using DNA dependant DNA polymerase Initiation & elongation commences Denature & anneal labelled primer * * dCTP dGTP dATP dTTPdCTP dATP dTTPdATP * * DNA template ddCTP

2. Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * DNA template ddCTP

3. Sequencing Using DNA dependant DNA polymerase Elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

4. Sequencing Using DNA dependant DNA polymerase Elongation continues for remaining molecules ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

5. Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

6. Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP * * ddCTP DNA template ddCTP

7. Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP * * ddCTP DNA template ddCTP

8. Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

9. Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

10. Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

11. Sequencing Using DNA dependant DNA polymerase Multiple products of different length terminated by A dideoxyCTP Theses molecules represent all the positiions within the complementary sequence where a “C” occurs Four separate reactions each terminated by a different dideoxy molecule would represent all bases Ordering these molecules by size allows us to determine the template sequence ddCTP * * * * * * *