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Published byMarilyn Stubblefield
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Toxinotyping of C. difficile strains B1 PCR fragment A3 PCR fragment PCR method for screening changes in PaLoc markers for toxinotyping B1 B2 B3 PL3 A1 A2 A3 PL4 tcdRtcdBtcdAtcdEtcdC 2 kb 2.5 kb3 kb2 kb3 kb3 kb 2.1 kb2 kb1.5 kb2 kb1.6 kb H N HcHcHH R H R Ec P X hhS E E PL2 PL1 M. Rupnik Institute of Public Health Maribor Medical Faculty, University of Maribor©
Types of A-B+ C. difficile strains
C. difficile typing – mostly used methods Ribotyping REA PFGE (Europe) (USA) (North America) PCR of 16S-23S rDNA intergenic spacer region 160 ribotypes Stubbs, JCM 1999 Bidet, JCM 2000 HindIII restriction of whole DNA >100 REA groups (Rea Types) Gerding D., Chicago, USA SmaI restriction of whole DNA no large international collection ©
Large clostridial toxins (LCT) C. difficile (TcdA, TcdB) C. sordellii (TcsH, TcsL) C. novyi (Tcn ) size ( kDa)size ( kDa) cytotoxicitycytotoxicity glycosyltransferasesglycosyltransferases autocatalytic proteolysisautocatalytic proteolysis ©
C. difficile pathogenicity locus (PaLoc) ©
Figure A1 – Relation between PFGE SmaI/Cfr9I clusters (80% similarity cut-off DICE/UPGMA) and emm sequence types for the 325 strains.
Profiling microbial communities with T-RFLP (terminal restriction fragment length polymorphism) Anne Fahy.
MYCOBACTERIOLOGY 2004 What laboratories are doing and where they are headed Current identification techniques Molecular Current identification techniques.
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TRAI and tariff reform The views expressed in this paper are those of the author and do not necessarily reflect the opinions of the ITU or its Membership.
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Nosocomial outbreaks Agnes Hajdu EpiTrain III, Jurmala, Latvia.
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1 Child Nutrition Services Understanding the Child Nutrition Tech Online Application/Agreement Step-By-Step REGION 3 Policy Update Meeting Thursday, February.
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Α-A26-39 rA26-39 rB ~ 270 kDa rB15-24 rA α-B ~ 308 kDa Supp. Figure 1: Toxins.
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