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Toxinotyping of C. difficile strains 1234567 B1 PCR fragment 12349556778 A3 PCR fragment PCR method for screening changes in PaLoc markers for toxinotyping.

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Presentation on theme: "Toxinotyping of C. difficile strains 1234567 B1 PCR fragment 12349556778 A3 PCR fragment PCR method for screening changes in PaLoc markers for toxinotyping."— Presentation transcript:

1 Toxinotyping of C. difficile strains B1 PCR fragment A3 PCR fragment PCR method for screening changes in PaLoc markers for toxinotyping B1 B2 B3 PL3 A1 A2 A3 PL4 tcdRtcdBtcdAtcdEtcdC 2 kb 2.5 kb3 kb2 kb3 kb3 kb 2.1 kb2 kb1.5 kb2 kb1.6 kb H N HcHcHH R H R Ec P X hhS E E PL2 PL1 M. Rupnik Institute of Public Health Maribor Medical Faculty, University of Maribor©

2 Types of A-B+ C. difficile strains

3 C. difficile typing – mostly used methods Ribotyping REA PFGE (Europe) (USA) (North America) PCR of 16S-23S rDNA intergenic spacer region 160 ribotypes Stubbs, JCM 1999 Bidet, JCM 2000 HindIII restriction of whole DNA >100 REA groups (Rea Types) Gerding D., Chicago, USA SmaI restriction of whole DNA no large international collection ©

4 Large clostridial toxins (LCT) C. difficile (TcdA, TcdB) C. sordellii (TcsH, TcsL) C. novyi (Tcn  ) size ( kDa)size ( kDa) cytotoxicitycytotoxicity glycosyltransferasesglycosyltransferases autocatalytic proteolysisautocatalytic proteolysis ©

5 C. difficile pathogenicity locus (PaLoc) ©


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