1. Review Game GENETIC ENGINEERING

2. Best Arnold character 1. 2. 3. 4.

3. DNA that is from two or more organisms is called… 1.Combinant DNA 2.Remade DNA 3.Recombinant DNA 4.Multiple DNA

4. Fastest Responders (in seconds) 4.91KAYLA COCKRELL 5.69ETHAN WHITE 6.09NORA WAARANIEMI 7EMILY TURNQUIST 7.6MICHAEL GIBBS

5. Today’s Topics Genetic Engineering Stem CellsGel Electrophoresis GMOs and Cloning Final Results

6. Genetic Engineering

7. Which of these is not an example of a vector used in genetic engineering? 1.Plasmid 2.Yeast 3.Virus 4.Bacteria

8. What is used to cut DNA? 1.Restriction Enzymes 2.DNA Ligase 3.Scissors 4.Recombinant Enzymes

9. If I wanted to produce human insulin using a transformed bacteria, what would I need to do first? 1.Recombine the DNA with a vector 2.Cut out the gene of interest 3.Isolate the gene of interest 4.Insert the gene into the bacteria

10. I have now made my Recombinant DNA. What must take place before I can clone the gene? 1.Transform the bacteria 2.Select and screen 3.Gene cloning 4.Use DNA Ligase

11. DNA fragments cut with EcoRI can be combined with DNA cut with 1.BamHI 2.HindIII 3.EcoRI 4.All of the above 5.1 & 2 6.2 & 3

12. Restriction fragments cut with a restriction enzyme have a single- stranded 4 base tail of AATT. What is this called? 1.Blunt end 2.Sticky end 3.Restriction tail 4.Ligase

13. How does the gene get cloned once it has been taken in by the bacteria? 1.Using PCR 2.Do more transformations 3.Bacterial reproduction 4.Gel electrophoresis

14. Once I have selected and screened the bacteria, I can now … 1.Extract the gene 2.Extract the protein 3.Give the bacteria to an ill person 4.All of these 5.1 & 2 6.1 & 3

15. Fastest Responders (in seconds) 11.89MICHAEL GIBBS 15.57KATHERINE SPURLOCK 18.42THOMAS NETTLETON 32.7SAMUEL KAUTZKY

16. Team Scores 63.33Terminator 53.75Kindergarten Cop 51.1Mr. Freeze 50Predator

17. GMOs and Cloning FASTEST FINGERFASTEST FINGER

18. Which is easier to clone? 1.Animals 2.Plants 3.Humans

19. Which of these is not a reason for making crops GMOs? 1.Better Taste 2.Drought Resistance 3.Pest Resistant 4.All of the above 5.Only 2 and 3

20. If I wanted to clone a sheep 1, using an egg from sheep 2, and having sheep 3 deliver the baby, where do I get the DNA from? 1.Sheep 1 2.Sheep 2 3.Sheep 3

21. The method of cloning described in the last slide is called? 1.Nuclear replacement 2.Somatic cell nuclear transplantation 3.Artificial twinning 4.Egg cell fertilization

22. Which type of cell can I get the DNA from? 1.Heart cell 2.Skin cell 3.Liver cell 4.Muscle cell 5.None of these 6.All of these

23. Fastest Responders (in seconds) 10.77NORA WAARANIEMI 11.11BRANDON LINGEN 12.1KATHERINE SPURLOCK 12.79KYLIE JOHNSON 19.38LIESE PETERSON

24. Team Scores 88.2Terminator 78.65Mr. Freeze 77.52Kindergarten Cop 75.77Predator

25. Gel Electrophoresis FASTEST FINGERFASTEST FINGER

26. Gel Electrophoresis does what? 1.DNA Fingerprinting 2.Paternity testing 3.Separates molecules by size 4.Uses electricity

27. Since everyone on the planet has different DNA, identical twins excluded, 1.No one has the same DNA fingerprint 2.Most people have a different DNA fingerprint 3.You are less likely to have the same DNA fingerprint as another

28. In RFLP analysis, with what do we mix our DNA samples before running them through a gel 1.DNA ligase 2.plasmids 3.Restriction enzymes 4.DNA polymerase

29. Who is the baby’s father? 1.Bob 2.Larry 3.Neither

30. Which direction would DNA move while being separated in gel electrophoresis? 1.Towards the positive 2.Towards the negative 3.Both directions depending on the person

31. Which DNA Fragment is shorter? 1.A 2.B

32. Who matches the blood stain? 1.Bob 2.Sue 3.John 4.Lisa

33. PCRs main function is to 1.Exponentially Amplify DNA 2.Make a few copies of DNA 3.Catch criminals 4.Extract DNA

34. Fastest Responders (in seconds) 3.13MICHAEL GIBBS 5.69ERIN LOUWAGIE 6.01KEVIN STOWE 8.33NORA WAARANIEMI 8.58CALEB TROUTH

35. Team Scores 117.94Predator 117.78Terminator 110.98Mr. Freeze 102.19Kindergarten Cop

36. Stem Cells

37. Which of these can differentiate into the widest variety of cells? 1.Totipotent 2.Multipotent 3.Pluripotent

38. Embryonic stem cells are 1.Totipotent 2.Pluripotent 3.Multipotent

39. Where do most adult stem cells used in medicine come from? 1.Kidneys 2.Skin 3.Blood 4.Bone marrow

40. Adult stem cells are 1.Totipotent 2.Pluripotent 3.Multipotent

41. Which type of stem cell is created from a differentiated somatic cell? 1.Adult stem cells 2.Embryonic stem cells 3.Induced pluripotent stem cells

42. Fastest Responders (in seconds) 4.87MICHAEL GIBBS

43. Team Scores 142.76Mr. Freeze 137.94Predator 137.78Terminator 132.19Kindergarten Cop

44. Team MVP PointsTeamParticipant 179.91Mr. FreezeMICHAEL GIBBS 147.07PredatorERIN LOUWAGIE 151.84TerminatorTIFFANY LO 163.27Kindergarten CopEMILY TURNQUIST

45. Participant Scores 179.91MICHAEL GIBBS129.4TRISHA MORRISON 165.66BRANDON LINGEN126.43TAYLOR HAEFELE 163.27EMILY TURNQUIST120.2RISHA SINGH 151.84TIFFANY LO118.49KAYLA COCKRELL 150.67CALEB TROUTH118.43KYLIE JOHNSON 147.07ERIN LOUWAGIE115.6PETER KILIAN 141.37KATHERINE SPURLOCK 112.91PETER FOMIN 135.43LIESE PETERSON112.38KEVIN STOWE 132.76NORA WAARANIEMI103.77THOMAS NETTLETON 132.11ETHAN WHITE101.31LYDIA BOIKE