We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byAlexa Maher
Modified over 2 years ago
© SENSORIN Elicia Wong, PhD Revolutionary Technology in pH Measurement Mar 17-19, 2009
© SENSORIN L 80 L 400 L 2,000 L 10,000 L Upstream ProcessDownstream Process pH Control Why is it a problem in bioprocessing? Wide Applications: Why is it Important in bioprocessing? Pharmaceutical/biotechnology (medicine) Cosmetic (perfume) Food and beverage (wine, sugar) Water purification & wastewater treatment Petroleum/deep sea/outer space exploration Critical quality parameter for cell and bacterial growth and metabolic activity, productivity and consistency. Drifting Fouling Calibration
© SENSORIN Potentiometric pH measurement Outline Existing pH measurement technologies Optical pH measurement Glass electrode Ion-selective field effect transistor (ISFET) New technology Amperometric pH measurement
© SENSORIN External reference electrode Measuring electrode High impedance pH meter Glass frit Glass membrane Inner solution Metal wire Voltage difference builds up on the sides of the glass membrane due to difference between H + activities on both sides This voltage difference is measured with the help of an external reference electrode Often reference and measuring electrodes are combined in one single structure Fragile, wet storage, long term drift, requires frequent calibration Potentiometric Approach – Glass electrode Glass frit Inner solution External reference electrode High impedance pH meter Glass membrane Glass electrode
© SENSORIN Ion-Selective Field Effect Transistor Vgs Electron path Vds Drain Source Ion-selective interface Insulator External reference electrode Analyte solution Cathode (-)Anode (+) No current flows - -- Current flows H+H+ H+H+ H+H+ Four major components: Source (s), Drain (d), Ion- selective interface and Gate (g). Hydrogen ions at or near the ion-selective interface will cause a variable voltage potential (Vgs – Vds) Solid state device
© SENSORIN Photo- detector Optical pH Measurement System Photodetector, optical fiber, sensor Sensor = fluorophore on solid support An incident beam of light is passed to the sensor, where it interacts with the fluorophore. The emitted light intensity is pH dependent Keep in dark (photo- bleaching) Sensor Optical light guide Incident beam Emitted light
© SENSORIN Comparison Feature Glass Electrode ISFETOptical Robustness Glass (fragile)Solid state Calibration Required (frequently) Required (longer calibration interval) Required (factory+on site) Stability Drift Maintenance Intensive (wet storage) Easy (dry storage) Intensive (kept in dark (photo- bleaching) Temperature Range Wide Narrow pH range (in ranges) Biological Fouling Occur Sensitivity Low MediumGood
© SENSORIN pH-sensitive interface semiconductor Amperometric pH Sensor Counter electrode Internal reference Power supply i V H+H+ H+H+ H+H+ H+H+ VV ii pH-sensitive interface semiconductor H+H+ H+H+ H+H+ H+H+ VV ii pH-sensitive interface + Internal reference External reference electrode Counter electrode Reference electrode Analyte solution pH-sensitive interface
© SENSORIN Self-Calibrating pH 4 pH 7 pH 10 Internal reference = pH insensitive molecule pH sensitive molecules Internal reference
© SENSORIN Stability chemicallythermally stable Both the proprietary pH sensitive and insensitive molecules are chemically stable (wont leach) and thermally stable (able to withstand high temperature up to 250 C). No degradation in performance with continuous operation and after heat sterilization. Responses were observed after multiple autoclaving cycles (measurement performed after each autoclaving cycle) Continuous operation No degradation in signal with 2-day continuous operation after autoclaving Multiple Autoclaving
© SENSORIN Free from Biological Fouling pH detection relies purely on chemical interaction between hydrogen ion and sensing interface rather than membrane diffusion effect (glass electrode) Continuous measurement in medium containing living cells pH insensitive molecules pH sensitive molecules Without pH control Detect change in pH from pH 7.82* to 7.01* *pH verified by off-line pH measurements - H + H+H+ Clotting of glass membrane
© SENSORIN Summary Chart Feature Glass Electrode ISFETOptical Amperometric Sensor Robustness Glass (fragile) Solid state Calibration Required (frequently) Required (longer calibration interval) Required (factory) Self calibrated Stability Drift No drift Maintenance Intensive (wet storage) Easy (dry storage) Intensive (Kept in dark (photo- bleaching) Easy (dry storage) Temperature Range Wide NarrowWide pH range (in ranges)0-14 Biological Fouling Occur NO Sensitivity LowMediumGood Ideal for bioprocess pH measurement
INDUSTRIAL HYGIENE DIRECT-READING INSTRUMENTS FOR GASES, VAPORS, AND PARTICULATES UNIVERSITY OF HOUSTON - DOWNTOWN.
X – Ray methods of Analysis : X – Absorption spectroscopy. X – Ray Fluorescence Spectroscopy. X – Ray Diffractometry.
Electrochemical Sensors Electrochemical sensors are the most versatile and highly developed chemical sensors. They are divided into several types: Potentiometric.
LCD TFT LED-OLED CCD CMOS DISPLAY SYSTEMS AND PHOTOSENSORS (PART 2)
Vi verner liv, miljø og verdier... 1 Gas Detection Systems Sensor Operating Theory.
PEC – UET Lahore Dr. Shahid Naveed 14. Januar 2014 / Dr. –Ing Naveed Ramzan 1 Industrial Instrumentation Dr. –Ing. Naveed Ramzan.
RADIOGRAPHIC TESTING. Introduction This module presents information on the NDT method of radiographic inspection or radiography. Radiography uses penetrating.
Mark Ledger MD Agua Africa Mark Titus Product Manager HACH.
1 EC1313 – LINEAR INTEGRATED CIRCUITS Name : M.Venmathi Designation:Senior Lecturer Department:Electrical and Electronics Engineering College:Rajalakshmi.
Pressure Transducers پروژه درس سیستم های اندازه گیری
Interaction of radiation & matter ä Electromagnetic radiation in different regions of spectrum can be used for qualitative and quantitative information.
1 Topic 2 UV-VIS Absorption Spectroscopy. Electromagnetic spectrum 2
An Introduction to Structural Health Monitoring ISIS Educational Module 5: Produced by ISIS Canada.
COOLING TOWER. 8. COOLING TOWER Purpose of a cooling tower is to provide cool water at a certain temperature. The water used in a cooling tower is cooled.
FT-IR Instrument 1. 2 i)Dispersive spectrometers or ii)Fourier transform spectrometers. Most commercial instruments separate and measure IR radiation.
1 SITE REMEDIATION Pedro A. García Encina Department of Chemical Engineering University of Valladolid.
CCB-TOX Continuous Contamination Biomonitor Benefits, Functions & Applications Confidential & Preliminary.
Potentiometric Methods A.) Introduction : 1.) Potentiometric Methods: based on measurements of the potential of electrochemical cells in the absence of.
Portable Contamination Biomonitors PCB-TOX & PCB-TOX-SPOT Session I - Basics.
Vertex detector optimisation: status and strategies Introduction. Performance goals. Constraints due to machine and detector. Conceptual detector design.
Direct-Reading Gas Detection Instruments Presented by Michael D. Shaw.
Part 1 :Light and material بسم الله الر حمن الرحيم
ECSE-6290 Semiconductor Devices and Models II Spring, 2010 S. Sawyer 1-1 ECSE-6290 Semiconductor Devices and Models II Lecture 20: Laser Diodes Shayla.
Copyright © by Holt, Rinehart and Winston. All rights reserved. ResourcesChapter menu To View the presentation as a slideshow with effects select View.
SENSORS FOR BIOMEDICAL APPLICATION Engr. Hinesh Kumar (Lecturer)O.
LIGHT SENSORS Sensor Technology Metropolia University Kiia Tammi Jonathan Malangoni.
CCB-TOX Tutorial Sections 1-3 Technology & EWS Basics.
SIGRIST APPLICATIONS IN THE BEVERAGE INDUSTRY Turbidity and colour measurement in breweries.
TOPIC 3: CHEMISTRY of LIFE. 3.1: Chemical Elements and Water.
© 2016 SlidePlayer.com Inc. All rights reserved.