We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byAlexa Maher
Modified over 3 years ago
© SENSORIN Elicia Wong, PhD Revolutionary Technology in pH Measurement Mar 17-19, 2009
© SENSORIN L 80 L 400 L 2,000 L 10,000 L Upstream ProcessDownstream Process pH Control Why is it a problem in bioprocessing? Wide Applications: Why is it Important in bioprocessing? Pharmaceutical/biotechnology (medicine) Cosmetic (perfume) Food and beverage (wine, sugar) Water purification & wastewater treatment Petroleum/deep sea/outer space exploration Critical quality parameter for cell and bacterial growth and metabolic activity, productivity and consistency. Drifting Fouling Calibration
© SENSORIN Potentiometric pH measurement Outline Existing pH measurement technologies Optical pH measurement Glass electrode Ion-selective field effect transistor (ISFET) New technology Amperometric pH measurement
© SENSORIN External reference electrode Measuring electrode High impedance pH meter Glass frit Glass membrane Inner solution Metal wire Voltage difference builds up on the sides of the glass membrane due to difference between H + activities on both sides This voltage difference is measured with the help of an external reference electrode Often reference and measuring electrodes are combined in one single structure Fragile, wet storage, long term drift, requires frequent calibration Potentiometric Approach – Glass electrode Glass frit Inner solution External reference electrode High impedance pH meter Glass membrane Glass electrode
© SENSORIN Ion-Selective Field Effect Transistor Vgs Electron path Vds Drain Source Ion-selective interface Insulator External reference electrode Analyte solution Cathode (-)Anode (+) No current flows - -- Current flows H+H+ H+H+ H+H+ Four major components: Source (s), Drain (d), Ion- selective interface and Gate (g). Hydrogen ions at or near the ion-selective interface will cause a variable voltage potential (Vgs – Vds) Solid state device
© SENSORIN Photo- detector Optical pH Measurement System Photodetector, optical fiber, sensor Sensor = fluorophore on solid support An incident beam of light is passed to the sensor, where it interacts with the fluorophore. The emitted light intensity is pH dependent Keep in dark (photo- bleaching) Sensor Optical light guide Incident beam Emitted light
© SENSORIN Comparison Feature Glass Electrode ISFETOptical Robustness Glass (fragile)Solid state Calibration Required (frequently) Required (longer calibration interval) Required (factory+on site) Stability Drift Maintenance Intensive (wet storage) Easy (dry storage) Intensive (kept in dark (photo- bleaching) Temperature Range Wide Narrow pH range (in ranges) Biological Fouling Occur Sensitivity Low MediumGood
© SENSORIN pH-sensitive interface semiconductor Amperometric pH Sensor Counter electrode Internal reference Power supply i V H+H+ H+H+ H+H+ H+H+ VV ii pH-sensitive interface semiconductor H+H+ H+H+ H+H+ H+H+ VV ii pH-sensitive interface + Internal reference External reference electrode Counter electrode Reference electrode Analyte solution pH-sensitive interface
© SENSORIN Self-Calibrating pH 4 pH 7 pH 10 Internal reference = pH insensitive molecule pH sensitive molecules Internal reference
© SENSORIN Stability chemicallythermally stable Both the proprietary pH sensitive and insensitive molecules are chemically stable (wont leach) and thermally stable (able to withstand high temperature up to 250 C). No degradation in performance with continuous operation and after heat sterilization. Responses were observed after multiple autoclaving cycles (measurement performed after each autoclaving cycle) Continuous operation No degradation in signal with 2-day continuous operation after autoclaving Multiple Autoclaving
© SENSORIN Free from Biological Fouling pH detection relies purely on chemical interaction between hydrogen ion and sensing interface rather than membrane diffusion effect (glass electrode) Continuous measurement in medium containing living cells pH insensitive molecules pH sensitive molecules Without pH control Detect change in pH from pH 7.82* to 7.01* *pH verified by off-line pH measurements - H + H+H+ Clotting of glass membrane
© SENSORIN Summary Chart Feature Glass Electrode ISFETOptical Amperometric Sensor Robustness Glass (fragile) Solid state Calibration Required (frequently) Required (longer calibration interval) Required (factory) Self calibrated Stability Drift No drift Maintenance Intensive (wet storage) Easy (dry storage) Intensive (Kept in dark (photo- bleaching) Easy (dry storage) Temperature Range Wide NarrowWide pH range (in ranges)0-14 Biological Fouling Occur NO Sensitivity LowMediumGood Ideal for bioprocess pH measurement
South Fork Instruments Advances in pH/ORP Measurement – Polymeric Sensors Presented by John Daly South Fork Instruments.
Luminescence Dissolved Oxygen an optical and unique way (patent pending) of measuring Dissolved Oxygen eliminates nearly all disadvantages of traditional.
OFFLINE COMPOSITION MEASURING SENSORS PREPAIRED BY: 07-CHEM-66 1.
Fall 2014, Prof. JB Lee Ion Sensitive FET (ISFET) - What and Why?
Potentiometry and potentiometric measurements. potentiometer A device for measuring the potential of an electrochemical cell without drawing a current.
BASIC ELECTRONICS. CONTENTS Resistor Capacitor Diode Transistor.
Dr. Marc Madou, UCI, Winter 2012 Class V Potentiometric and Amperometric Sensors (I) Electrochemistry MAE-295.
Sensors and Electricity. What is a Sensor? A sensor is a device that: A sensor is a device that: 1) Measures a physical quantity 2) Converts this measurement.
Summary of Potentiometry: pH and Ion Selective Electrodes Potentiometric Sensors I = controlled at 0 Amps E eq is measured In general E eq = const –
POTENTIALS AND THERMODYNAMICS OF CELLS (1) POTENTIALS AND THERMODYNAMICS OF CELLS (1)
Chem. 133 – 2/12 Lecture. Announcements Lab Work –Supposed to Cover Set 2 Labs – but I probably won’t cover all and then will give an extra day for period.
Measurement of Dissolved Oxygen with a Luminescence-based Oxygen Quenching Sensor By Cary B. Jackson, Ph.D. Hach Company.
Potentiometry. pH is a Unit of Measurement pH = Power of Hydrogen (H + ) Defined as the Negative Logarithm of Hydrogen Ion Activity pH = log (1/H.
Semiconductor Light Detectors ISAT 300 Foundations of Instrumentation and Measurement D. J. Lawrence Spring 1999.
Panel 3D = XML file pointer 08/09/20091 LHCb calorimeter meeting (jean-luc PANAZOL)
Dr. Marc Madou Class II. Electrochemistry Background (I) Winter 2009 BIOMEMS.
MOCT(Magneto Optic Current Transduser). INTRODUCTION An accurate electric current transducer is a key component of any power system instrumentation. To.
Electrodes and Potentiometry Introduction 1.)Potentiometry Use of Electrodes to Measure Voltages that Provide Chemical Information - Various electrodes.
Contents : CONSTRUCTION PRINCIPLE OF OPERATION CHARACTERISTICS ADVANTAGES DISADVANTAGES APPLICATION.
Carl Wirdak Occidental Petroleum Corporation GEMI Survey Water Use, Issues & Management March 2001.
Biomedical Electrodes, Sensors, and Transducers from: Chaterjee, Biomedical Instrumentation, chapter 6 © D. J. McMahon 2014 rev
LCD TFT LED-OLED CCD CMOS DISPLAY SYSTEMS AND PHOTOSENSORS (PART 2)
Atomic Absorption Spectrophotometer Hossein Piri Hossein Piri Department of Biochemistry and Genetics, School of Medicine, Qazvin University of Medical.
MLAB 2401: C LINICAL C HEMISTRY K ERI B ROPHY -M ARTINEZ Designs in Instrumentation Electrochemistry.
Nelson Research, Inc – N. 88 th St. Seattle, WA USA aol.com Bubble Detection Sensors for Non-conducting Liquids Craig.
1 Microelectronics Processing Course - J. Salzman - Jan Microelectronics Processing Oxidation.
WORKING PRINCIPLE OF DO AND pH PROBE Prepared by: Tan See Yean Yuganesh a/l Murugiah Jelita Bungin Nazzira Md Zin.
Vi verner liv, miljø og verdier... 1 Gas Detection Systems Sensor Operating Theory.
Electrochemical Cells in Actions Batteries and Fuel Cells Chapter 15.
Home Reading Skoog et al. Fundamental of Analytical Chemistry. Chapters 18, 19, 21.
A WIRELESS PASSIVE SENSOR FOR TEMPERATURE COMPENSATED REMOTE PH MONITORING IEEE SENSORS JOURNAL VOLUME 13, NO.6, JUNE 2013 WEN-TSAI SUNG, YAO-CHI HSU Ching-Hong.
EKT 314/4 WEEK 4 : CHAPTER 2 TRANSDUCERS ELECTRONIC INSTRUMENTATION.
Section 2 Current. Voltage and Current Electrical Potential Energy: the ability to move an electrical charge from one point to another. Depends on position.
Information Security of Embedded Systems : Embedded Systems Design Prof. Dr. Holger Schlingloff Institut für Informatik und Fraunhofer FIRST.
Basic electronic components A review Made by Yulia I. Isakova As a template for students’ presentations Tomsk 2015.
Lasers and Optics By Adam Abawi. Lasers vs. Light A laser differs from other sources of light in that it emits light in a narrow straight line A laser.
Biosensors Dr. Bhavesh Patel Principal V.P. and R.P.T.P. Science College, Vallabh Vidyanagar -
DC Analysis Representation of diode into three models Ideal case – model 1 with V = 0 Piecewise linear model 2 with V has a given value Piecewise linear.
PH THEORY What is it pH?What is it pH?/How is pH Measured?/What Equipment is Required to Measure pH?/How is a pH Measurement Device Calibrated?/Why is.
WEEK ONE TOPIC: ELECTRONICS SOLID STATE MATERIALS CONDUCTORS INSULATORS SEMICONDUCTORS.
ElectronicsCRO 1 Cathode Ray Oscilloscope (CRO) Structure of CRO Vertical deflection of the spot Next Slide Horizontal deflection of the spot Common patterns.
Section 2.1 Controlling the Flow of Electrical Current.
1 Chapter 9. PN-junction diodes: Applications Diode applications: –Rectifiers –Switching diodes –Zener diodes –Varactor diodes (Varactor = Variable reactance)
FLAME SPECTROSCOPY The concentration of an element in a solution is determined by measuring the absorption, emission or fluorescence of electromagnetic.
NSF-ITR Meeting Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses.
M. Mayer SEWG Fuel Retention June Sample Analysis for TS, AUG and JET: Depth Profiling of Deuterium M. Mayer Max-Planck-Institut für Plasmaphysik,
Chapter 17 Electrochemistry The study of the interchange of chemical and electrical energy. Primarily concerned with two processes: –Oxidation-reduction.
1 Chapter 5 Sensors and Detectors A detector is typically the first stage of a communication system. Noise in this stage may have significant effects on.
Automation Solutions for Ladle Gate Applications SEAISI 2009, Kuala Lumpur.
© 2017 SlidePlayer.com Inc. All rights reserved.