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Internal Parasites Lab INAG 120 – Equine Health Management November 16, 2011.

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Presentation on theme: "Internal Parasites Lab INAG 120 – Equine Health Management November 16, 2011."— Presentation transcript:

1 Internal Parasites Lab INAG 120 – Equine Health Management November 16, 2011

2 Performing Fecal Egg ID/Count Fecal egg counts are useful for: Fecal egg counts are useful for: Routine monitoring of parasite status Routine monitoring of parasite status Identification of infected horses for targeted treatment Identification of infected horses for targeted treatment Investigation of the parasite status of a new horse Investigation of the parasite status of a new horse Investigation of colic and weight loss Investigation of colic and weight loss

3 Benefits of Fecal Egg ID/Count If parasites known, targeted deworming can be done If parasites known, targeted deworming can be done Lowers cost Lowers cost Less chemicals used in horses body Less chemicals used in horses body Horses with heavy parasite burdens can be treated before they suffer from a parasite associated disease Horses with heavy parasite burdens can be treated before they suffer from a parasite associated disease Efficacy of worm control programs can be monitored Efficacy of worm control programs can be monitored

4 Performing Fecal Egg ID/Count Limitations of test Limitations of test Does not detect immature or encysted stages of strongyle parasites Does not detect immature or encysted stages of strongyle parasites Does not discriminate between large and small strongyles Does not discriminate between large and small strongyles Tapeworms often do not appear Tapeworms often do not appear Results not well correlated with total parasite burden Results not well correlated with total parasite burden

5 Types of Tests Direct Flotation Method Direct Flotation Method Qualitative Qualitative Shows what kind of eggs are present Shows what kind of eggs are present Fecal Egg Count Reduction Test (FECRT) Fecal Egg Count Reduction Test (FECRT) Also known as McMaster Method Also known as McMaster Method Quantitative Quantitative Shows how many eggs are present in one gram of feces (EPG) Shows how many eggs are present in one gram of feces (EPG)

6 Performing Fecal Count Tests Supplies Needed Supplies Needed Fresh feces Fresh feces Fecal flotation solution Fecal flotation solution Stirrer Stirrer Cheese cloth Cheese cloth Transfer pipette to syringe Transfer pipette to syringe Fecal egg count kit Fecal egg count kit 100X microscope 100X microscope

7 Performing Fecal Count Test Step by Step Procedure Obtain fresh sample of feces, free of shavings or other debris Obtain fresh sample of feces, free of shavings or other debris Add flotation solution to line A of vial provided by the kit Add flotation solution to line A of vial provided by the kit Add subsample of fresh feces to bring volume up to Line B of vial Add subsample of fresh feces to bring volume up to Line B of vial Using stirrer, stir/mix thoroughly for seconds Using stirrer, stir/mix thoroughly for seconds

8 Performing Fecal Count Test Filter solution through a cheese cloth into a new vial to remove large fecal particles Filter solution through a cheese cloth into a new vial to remove large fecal particles Immediately draw up sample from vial into syringe or transfer pipette Immediately draw up sample from vial into syringe or transfer pipette Place transfer pipette tip at the edge of the slide and discharge sufficient sample between the upper and lower slides to fill the chamber. Place transfer pipette tip at the edge of the slide and discharge sufficient sample between the upper and lower slides to fill the chamber. Repeat steps to fill the second chamber Repeat steps to fill the second chamber

9 Performing Fecal Count Tests Place slide on microscope stage Place slide on microscope stage View slide under 100x magnification View slide under 100x magnification Focus on the slides grid lines. The eggs will have floated to the top of the chamber in effect sticking to the underside of the top slide Focus on the slides grid lines. The eggs will have floated to the top of the chamber in effect sticking to the underside of the top slide Starting from the outside corner, count all eggs inside each grid utilizing grid lines in an orderly fashion (from left to right). Starting from the outside corner, count all eggs inside each grid utilizing grid lines in an orderly fashion (from left to right). Do not count eggs outside of the grid lines Do not count eggs outside of the grid lines

10 Slide Gridlines

11 Performing Fecal Test

12

13 Calculation Record the number of eggs in each of the grids: Record the number of eggs in each of the grids: Grid 1: _________________ eggs Grid 1: _________________ eggs Grid 2: _________________ eggs Grid 2: _________________ eggs Calculate eggs per gram feces (EPG): Calculate eggs per gram feces (EPG): Grid 1 eggs + Grid 2 eggs = ____________ eggs Grid 1 eggs + Grid 2 eggs = ____________ eggs Sum x 25 = ______________ eggs per gram Sum x 25 = ______________ eggs per gram

14 Deciphering the Egg Counts Infestation level Fecal Eggs/Gram Mild < 200 Moderate Severe > 500

15 Strongyles Small strongyle eggs (cyathostomes) have a characteristic strongylid appearance, meaning they are oval and about 80 by 50 microns, have a thin complete eggshell, and contain a developing morula.

16 Ascarids Round with thick outer layer

17 Pinworms This egg has a thicker eggshell with a plug on one end, is often flattened on one side, and already contains a developing larva.

18 Tapeworms D-shaped

19 Strongyles TapewormsPinworms Ascarids


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