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Internal Parasites Lab

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Presentation on theme: "Internal Parasites Lab"— Presentation transcript:

1 Internal Parasites Lab
INAG 120 – Equine Health Management November 16, 2011

2 Performing Fecal Egg ID/Count
Fecal egg counts are useful for: Routine monitoring of parasite status Identification of infected horses for targeted treatment Investigation of the parasite status of a new horse Investigation of colic and weight loss

3 Benefits of Fecal Egg ID/Count
If parasites known, targeted deworming can be done Lowers cost Less chemicals used in horse’s body Horses with heavy parasite burdens can be treated before they suffer from a parasite associated disease Efficacy of worm control programs can be monitored 

4 Performing Fecal Egg ID/Count
Limitations of test Does not detect immature or encysted stages of strongyle parasites Does not discriminate between large and small strongyles Tapeworms often do not appear Results not well correlated with total parasite burden

5 Types of Tests Direct Flotation Method
Qualitative Shows what kind of eggs are present Fecal Egg Count Reduction Test (FECRT) Also known as McMaster Method Quantitative Shows how many eggs are present in one gram of feces (EPG)

6 Performing Fecal Count Tests
Supplies Needed Fresh feces Fecal flotation solution Stirrer Cheese cloth Transfer pipette to syringe Fecal egg count kit 100X microscope

7 Performing Fecal Count Test Step by Step Procedure
Obtain fresh sample of feces, free of shavings or other debris Add flotation solution to line A of vial provided by the kit Add subsample of fresh feces to bring volume up to Line B of vial Using stirrer, stir/mix thoroughly for seconds

8 Performing Fecal Count Test
Filter solution through a cheese cloth into a new vial to remove large fecal particles Immediately draw up sample from vial into syringe or transfer pipette Place transfer pipette tip at the edge of the slide and discharge sufficient sample between the upper and lower slides to fill the chamber. Repeat steps to fill the second chamber

9 Performing Fecal Count Tests
Place slide on microscope stage View slide under 100x magnification Focus on the slide’s grid lines. The eggs will have floated to the top of the chamber in effect sticking to the underside of the top slide Starting from the outside corner, count all eggs inside each grid utilizing grid lines in an orderly fashion (from left to right). Do not count eggs outside of the grid lines

10 Slide Gridlines

11 Performing Fecal Test

12 Performing Fecal Test

13 Calculation Record the number of eggs in each of the grids:
Grid 1: _________________ eggs Grid 2: _________________ eggs Calculate eggs per gram feces (EPG): Grid 1 eggs + Grid 2 eggs = ____________ eggs Sum x 25 = ______________ eggs per gram

14 Deciphering the Egg Counts
Infestation level Fecal Eggs/Gram Mild < 200 Moderate Severe > 500

15 Strongyles Small strongyle eggs (cyathostomes) have a characteristic strongylid appearance, meaning they are oval and about 80 by 50 microns, have a thin complete eggshell, and contain a developing morula.

16 Ascarids Round with thick outer layer

17 Pinworms This egg has a thicker eggshell with a plug on one end, is often flattened on one side, and already contains a developing larva.

18 Tapeworms D-shaped

19 Strongyles Ascarids Pinworms Tapeworms

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