7 Colony Statistics for Plating Density of 2 x 106 (n=12 chambers) Mouse Bone Stem CellsColony Statistics for Plating Density of 2 x 106 (n=12 chambers)WTKONumber Colonies168 (35)82 (37)Number Cells/colony71 (102)56 (133)Size Colony (mm2)0.214 (0.343)0.161 (0.343)Cell Density (%)15.6 (6.7)17.0 (8.5)AP- Pos Staining (%)32.7 (23.7)22.8 (24.1)
8 Introduction - Micro-CT Imaging Micro-CT is a high resolution version of X-ray tomographic imaging (resolution = microns). It has primarily been used to image ex vivo bone core specimens. And more recently been used to image various bone structures in vivo in small animal models.
9 Introduction –Micro-CT Imaging DetectorX-raysourceObjectrotatesincreased scanning speedmore efficient use of x-raysreduced doseCone-Beam Micro-CT
10 CCF Micro-CT Imaging System X-raySourceRotationalStageImage Intensifier & CCD camera4 micron spot size X-raykV, mA3-field II (5, 7, 9 inch FOV)2k x 2k 12-bit CCD camera7 - axis positioning system
11 Micro-CT Imaging Applications Bone trabecular morphometryiliac crest, calcaneous, femoral headTissue engineeringBone density and location of new bone formation in HA and PCL bone tissue scaffoldsIn-vivo small animal imagingLongitudinal evaluation of callus and bone volume in in vivo bone fracture/healing modelMouse PhenotypingMorphometric analysis of skeletal structure in metalloprotease knockout mice