1. Estimation of Carbohydrate by Anthrone Method
The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.

2. * Principle
H2SO4 +
Furfural
Anthrone
Blue Green
Complex

3. Materials 1. Anthrone reagent (0.2% in conc. H2SO4 ).
2. Glucose (10mg/100ml).
3. Colorimeter or spectrophotometer.

4. COOL to Room Temperature Read the Optical Density at 620 nm
Tube # Blank St unk.
10 min. 60 30 15 45 5 10 20 25 35 40 50 55
Boiling Water Bath
Glucose (ml)
D. W. (ml)
Anthrone (ml)
Mix Well
COOL to Room Temperature
Read the Optical Density at 620 nm

5. * Calculations CUn. = A Un. × C St.
Absorbance of Unknown Absorbance of Standard =
Concentration of Unknown Concentration of Standard
CUn. = A Un. × C St.
A St.
Samar A. Damiati