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Estimation of Carbohydrate by Anthrone Method The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates,

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Presentation on theme: "Estimation of Carbohydrate by Anthrone Method The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates,"— Presentation transcript:

1 Estimation of Carbohydrate by Anthrone Method The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.

2 * Principle H 2 SO 4 + Furfural Anthrone Blue Green Complex

3 Materials 1. Anthrone reagent (0.2% in conc. H 2 SO 4 ). 2. Glucose (10mg/100ml). 3. Colorimeter or spectrophotometer.

4 Glucose (ml) Tube # Blank St. unk. D. W. (ml) Anthrone (ml) COOL to Room Temperature Mix Well 10 min. Read the Optical Density at 620 nm Boiling Water Bath

5 * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard C Un. = A Un. × C St. A St. Samar A. Damiati


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