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* Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method.

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Presentation on theme: "* Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method."— Presentation transcript:

1 * Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method

2 Materials Phosphovanillin reagent Sulfuric acid Standard solution of cholesterol (1g/100 ml acetone) Standard solution of olive oil

3 CholesterlOlive oilBlank 20µ D.W. 20µ st. 20µ unk. 20µ st. 20µ unk. H 2 SO 4 (ml) 0.2 0.2 0.2 0.2 0.2 Mix Well on a vortex mixer 60 30 15 45 5 10 20 2535 40 50 55 10 min. COOL in water at Room Temperature Phosphovanillin 10 10 10 10 10 (ml) Mix Well Incubate at 37ºC in water bath 15min. St.Unk.St.Unk. Read the Optical Density at 540 nm

4 * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard C Un. = A Un. × C St. A St. Samar A. Damiati


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