Presentation is loading. Please wait.

Presentation is loading. Please wait.

* Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method.

Similar presentations


Presentation on theme: "* Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method."— Presentation transcript:

1 * Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method

2 Materials Phosphovanillin reagent Sulfuric acid Standard solution of cholesterol (1g/100 ml acetone) Standard solution of olive oil

3 CholesterlOlive oilBlank 20µ D.W. 20µ st. 20µ unk. 20µ st. 20µ unk. H 2 SO 4 (ml) Mix Well on a vortex mixer min. COOL in water at Room Temperature Phosphovanillin (ml) Mix Well Incubate at 37ºC in water bath 15min. St.Unk.St.Unk. Read the Optical Density at 540 nm

4 * Calculations Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard C Un. = A Un. × C St. A St. Samar A. Damiati


Download ppt "* Principle + Carbonium ions H 2 SO 4 LIPIDS Vanillin phosphate ester Purple Complex + Estimation of Total Lipids by Colorimetric Method."

Similar presentations


Ads by Google