1. THINGS TO CONSIDER Patrick Rafter Minuteman High School

2. General Safety Considerations Biological/Biohazards-A definition Biological Hazards Safety Considerations Bio-Safety Tips Safe or what?-Read the MSDS Aseptic Technique http://www.123rf.com/

3. Lab Layout Escape Plan Fire Safety Equipment Eye Wash and Shower MSDS Books Chemical Storage Containers (Acid/Base/Explosion Proof/Refrigeration/Freezer/Waste Storage) Spill Kits/Sharps/Broken Glass Safety Posters/DVD/Worksheets Safety Contract Biological/Biohazard

4. Biohazards are infectious agents or hazardous biological materials that present a risk or potential risk to the health of humans, animals or the environment. (http://biosafety.ucla.ed u/bio_hazard_def.html) http://en.wikipe dia.org/wiki/Bio logical_hazard

5. They multiply rapidly They may mutate into new forms They may carry and transfer resistance They may cause illness or death We may be their host We cannot see them They may invade or adversely effect the environment They may have chronic, cumulative and long term effects http://www.123rf.com/

6. Pre-read safety material MSDS and disposal tips Wear PPE (gloves, lab-coat, safety eye-ware) Sharp containers Double bagging in Biohazard bins 10% Bleach and 70% IPA BSCII hoods Autoclave http://en.wikipedia.or g/wiki/Laminar_flow_ cabinet

7. Safe DNA dye Binds DNA-What about your DNA? Preservative solutions Not so safe according to MSDS Think Safety First Always read the MSDS Look-up required PPE No information on many health effects-beware http://www.123rf.com/

8. Develop Lab Rules Train Lab workers for safety Create SOPs for Aseptic Technique Workspace pre-sterilization Donning of PPE Use Kill Jars-10% Bleach Dispose of contaminated items in biohazard trash Autoclave Biohazard Trash Sterilize workspace after use with 70% IPA Removal and disposal or cleaning of PPE http://nobel.sc as.bcit.ca/debe ck_pt/science/s afety.htm

9. Biosafety in Microbiological and Biomedical Laboratories 5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf American Biological Safety Association http://www.absa.org/http://www.absa.org/ CDC http://www.cdc.gov/biosafety/http://www.cdc.gov/biosafety/ NIH http://www.nih.gov/http://www.nih.gov/ NIH Guidelines for recombinant DNA research http://oba.od.nih.gov/oba/rac/Guidelines/NIH_Guidelines.pdf http://oba.od.nih.gov/oba/rac/Guidelines/NIH_Guidelines.pdf BMBL Appendix A – Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_ appendixA.pdf http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_ appendixA.pdf Royalty Free Stock Images http://www.123rf.com/http://www.123rf.com/