Presentation is loading. Please wait.

Presentation is loading. Please wait.

Mikko I. Mäyränpää, MD, PhD, Julia A

Published byDemetrio Meloni Modified over 5 years ago

Similar presentations

Presentation on theme: "Mikko I. Mäyränpää, MD, PhD, Julia A"— Presentation transcript:

1 Mast cells associate with neovessels in the media and adventitia of abdominal aortic aneurysms 
Mikko I. Mäyränpää, MD, PhD, Julia A. Trosien, MSc, Vincent Fontaine, MD, PhD, Maggie Folkesson, MSc, Monsur Kazi, MD, PhD, Per Eriksson, PhD, Jesper Swedenborg, MD, PhD, Ulf Hedin, MD, PhD  Journal of Vascular Surgery  Volume 50, Issue 2, Pages (August 2009) DOI: /j.jvs Copyright © 2009 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Photomicrograph showing double-staining of a normal aorta (A) and an AAA wall without a thrombus (B; NTH AAA) and a thrombus-covered wall (C; TH AAA) for endothelial cells (CD31/CD34, AEC, red) and MCs (tryptase, nickel-DAB, black), as described in the methods. Arrows indicate MCs and arrowheads indicate endothelial cells in neovessels in the adventitia and media of the aneurysm wall. Inset in (A) shows the luminal endothelium and subendothelial MCs with higher magnification. Scale bars 300 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Redistribution of mast cells from intima to media during the progression AAA formation is seen in (A, media) and (B, intima). The proportional thickness of an aortic wall occupied by neovessels increases markedly in AAA samples compared with normal control aortas (C). Dots representing individual samples may overlap. Comparisons between controls and AAA samples (NTH or TH) were done with a Mann-Whitney U test. Paired comparisons with NTH and TH aneurysm samples were calculated using a Wilcoxon signed-rank test. Diamonds and vertical bars represent means and 95% confidence intervals. C, Microscopically normal aortic control specimens, AAA, abdominal aortic aneurysm; NTH, nonthrombotic AAA; TH, thrombus-covered AAA. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

4 Fig 3 mRNA expression of genes associated with neovascularization and endothelial cells as measured by quantitative real-time PCR in the control aortas and aneurysm wall segments. The values are arbitrary units after correction for RPLP0 expression. Comparisons between controls and AAA samples (NTH or TH) were done with a Mann-Whitney U test. Paired comparisons with NTH and TH aneurysm samples were calculated using a Wilcoxon signed-rank test. NTH, Nonthrombotic AAA; TH, thrombus-covered AAA; AAA, abdominal aortic aneurysm; PDGFD, platelet-derived growth factor D; VE-cadherin, vascular endothelial cadherin; ET-1, endothelin-1; FLT-1, FMS-related tyrosine kinase-1; VEGF, vascular endothelial growth factor. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

5 Fig 4 mRNA expression of genes associated with smooth muscle cells and mast cells as measured by quantitative real-time PCR in the control aortas and aneurysm wall segments. The values are arbitrary units after correction for RPLP0 or TBP expression. Comparisons between controls and AAA samples (NTH or TH) were done with a Mann-Whitney U test. Paired comparisons with NTH and TH aneurysm samples were calculated using a Wilcoxon signed-rank test. NTH, Nonthrombotic AAA; TH, thrombus-covered AAA; AAA, abdominal aortic aneurysm; RPLP0, ribosomal phosphoprotein large P0; TBP, TATA box-binding protein. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Representative confocal microscope image showing vascular endothelial growth factor (VEGF) (green) and tryptase (red) double-stained mast cells (MC) in human aneurysmatic aortic media. Also many other cells in the inflammatory infiltrates of aneurysms stained positively for VEGF. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Download ppt "Mikko I. Mäyränpää, MD, PhD, Julia A"

Similar presentations

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 
Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,

Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,
Hyperglycemia limits experimental aortic aneurysm progression

Hyperglycemia limits experimental aortic aneurysm progression
Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,
Chang M. He, MSc, Margot R. Roach, MD, PhD  Journal of Vascular Surgery 

Chang M. He, MSc, Margot R. Roach, MD, PhD  Journal of Vascular Surgery 
Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 

Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects 
Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,

Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft  Hongshan Zhong, MD, Osamu Matsui, MD,

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft  Hongshan Zhong, MD, Osamu Matsui, MD,
Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft  Hongshan Zhong, MD, Osamu Matsui, MD,

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft  Hongshan Zhong, MD, Osamu Matsui, MD,
Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis 

Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis 
Histologic analysis of stent graft oversizing in the thoracic aorta

Histologic analysis of stent graft oversizing in the thoracic aorta
The pro-inflammatory and chemotactic cytokine microenvironment of the abdominal aortic aneurysm wall: A protein array study  Rachel K. Middleton, BSc,

The pro-inflammatory and chemotactic cytokine microenvironment of the abdominal aortic aneurysm wall: A protein array study  Rachel K. Middleton, BSc,
The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,

The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,
Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V

Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V
Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,
Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,
Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 

Similar presentations

Ads by Google