Presentation is loading. Please wait.

Presentation is loading. Please wait.

Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V

Published byἈντιόπη Παπάζογλου Modified over 5 years ago

Similar presentations

Presentation on theme: "Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V"— Presentation transcript:

1 Cellular localization of matrix metalloproteinases in the abdominal aortic aneurysm wall 
Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V. Scholes, MD, Yutaka Ogata, MD*, Hideaki Nagase, PhD, M.David Tilson, MD  Journal of Vascular Surgery  Volume 20, Issue 5, Pages (November 1994) DOI: /S (94) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Identification of cells expressing MMP-9 by immunohistochemistry. Cell-specific staining is brown in sections incubated with sheep anti-human MMP-9 IgG and detected with HRP and diaminobenzidine shown by arrows in panel A. Nuclei are counterstained with toluidine blue. Panel B is fluorescence photomicrograph of same field as shown in panel A. Specimen was dual-labeled with monoclonal mouse anti-human CD14 IgG and detected with FITC-labeled anti-mouse IgG. Arrows indicate cells that are positive for both anti-MMP-9 IgG and anti-CD14 IgG. Black bars indicate 10 μm in magnified field. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 1 Identification of cells expressing MMP-9 by immunohistochemistry. Cell-specific staining is brown in sections incubated with sheep anti-human MMP-9 IgG and detected with HRP and diaminobenzidine shown by arrows in panel A. Nuclei are counterstained with toluidine blue. Panel B is fluorescence photomicrograph of same field as shown in panel A. Specimen was dual-labeled with monoclonal mouse anti-human CD14 IgG and detected with FITC-labeled anti-mouse IgG. Arrows indicate cells that are positive for both anti-MMP-9 IgG and anti-CD14 IgG. Black bars indicate 10 μm in magnified field. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 2 Representative negative control section. AAA tissue sections were incubated with nonimmune sheep serum and processed with HRP and diaminobenzidine. Nuclei are counterstained with toluidine blue. Black bar on lower right is 10 μm in magnified field. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 3 MMP-1, MMP-3, and uPA protein localization by immunohistochemistry. Sheep anti-human MMP-1 IgG labels endothelial cells (panel A) and mesenchymal cells (panel B). MMP-3 detected with sheep antihuman MMP-3 serum in mononuclear cells morphologically determined to be macrophages is shown in panels C and D. With rabbit anti-human uPA serum, uPA is detected in macrophage-like mononuclear cells in media and adventitia of aneurysmal aortic wall shown in panels E and F. Cells with positive results are indicated by arrows. Typical results with control tissues are shown at top of figure. Black bars are 10 μm in magnified fields. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 3 MMP-1, MMP-3, and uPA protein localization by immunohistochemistry. Sheep anti-human MMP-1 IgG labels endothelial cells (panel A) and mesenchymal cells (panel B). MMP-3 detected with sheep antihuman MMP-3 serum in mononuclear cells morphologically determined to be macrophages is shown in panels C and D. With rabbit anti-human uPA serum, uPA is detected in macrophage-like mononuclear cells in media and adventitia of aneurysmal aortic wall shown in panels E and F. Cells with positive results are indicated by arrows. Typical results with control tissues are shown at top of figure. Black bars are 10 μm in magnified fields. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer  Ming-Chuan Wang, PhD, Ying-Hua Chang,

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer  Ming-Chuan Wang, PhD, Ying-Hua Chang,
Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.

Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.
Human adult endothelial cell growth in culture

Human adult endothelial cell growth in culture
Expression of molecular mediators of apoptosis and their role in the pathogenesis of lower-extremity varicose veins  Enrico Ascher, MD, Theresa Jacob,

Expression of molecular mediators of apoptosis and their role in the pathogenesis of lower-extremity varicose veins  Enrico Ascher, MD, Theresa Jacob,
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.
Hyperglycemia limits experimental aortic aneurysm progression

Hyperglycemia limits experimental aortic aneurysm progression
Effect of tissue plasminogen activator on vascular smooth muscle cells

Effect of tissue plasminogen activator on vascular smooth muscle cells
Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.

Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.
Chang M. He, MSc, Margot R. Roach, MD, PhD  Journal of Vascular Surgery 

Chang M. He, MSc, Margot R. Roach, MD, PhD  Journal of Vascular Surgery 
Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,

Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer  Ming-Chuan Wang, PhD, Ying-Hua Chang,

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer  Ming-Chuan Wang, PhD, Ying-Hua Chang,
Indomethacin inhibits expansion of experimental aortic aneurysms via inhibition of the cox2 isoform of cyclooxygenase  Manuel Miralles, MD, William Wester,

Indomethacin inhibits expansion of experimental aortic aneurysms via inhibition of the cox2 isoform of cyclooxygenase  Manuel Miralles, MD, William Wester,
Orally administered dipeptidyl peptidase-4 inhibitor (alogliptin) prevents abdominal aortic aneurysm formation through an antioxidant effect in rats 

Orally administered dipeptidyl peptidase-4 inhibitor (alogliptin) prevents abdominal aortic aneurysm formation through an antioxidant effect in rats 
Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD 

Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD 
Inducible nitric oxide synthase is present in human abdominal aortic aneurysm and promotes oxidative vascular injury  Jian Zhang, MD, Jan Schmidt, MD,

Inducible nitric oxide synthase is present in human abdominal aortic aneurysm and promotes oxidative vascular injury  Jian Zhang, MD, Jan Schmidt, MD,
In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta  William D. McMillan,

In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta  William D. McMillan,
Association of intraluminal thrombus in abdominal aortic aneurysm with local hypoxia and wall weakening  David A. Vorp, PhDa,b,c, Paul C. Lee, MDa, David.

Association of intraluminal thrombus in abdominal aortic aneurysm with local hypoxia and wall weakening  David A. Vorp, PhDa,b,c, Paul C. Lee, MDa, David.

Similar presentations

Ads by Google