1. Optical labelling by two‐photon excitation.
Optical labelling by two‐photon excitation. (A) HEK‐293 cultures expressing KikGR were imaged using an FV500 Olympus confocal microscope equipped with Ar (488 nm) and He/Ne (543 nm) lasers. Transverse images were reconstituted using the Fluoview software. (B) KikGR photoconversion was restricted to only one cell after a Ti‐Sapphire ultra‐short pulse laser (760 nm, 80 MHz). Scale bar, 10 μm.
Hidekazu Tsutsui et al. EMBO Rep. 2005;6:
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