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SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

Published byLeander Langenberg Modified over 5 years ago

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Presentation on theme: "SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody."— Presentation transcript:

1 SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody after cross‐linking with DSP was clearly detected in wild‐type (WT) mouse neonatal cardiomyocytes. SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody after cross‐linking with DSP was clearly detected in wild‐type (WT) mouse neonatal cardiomyocytes. Co‐immunoprecipitated TLR9 was abolished in cardiomyocytes from TLR9−/− mice, confirming the specificity of the detecting antibody. Each lane contains proteins immunoprecipitated from 5 × 106 cells. The overexpressed TLR9 colocalized with SERCA2 at the ER/SR. TLR9‐HA‐FLAG was expressed in cardiomyocytes and labelled with anti‐HA antibody (green) in addition to SERCA2 (red) antibody. Endogenous TLR9 colocalized with SERCA2 in cardiomyocytes. Rat neonatal cardiomyocytes were stained with TLR9 (green) and SERCA2 (red) antibodies at 15 min after CpG‐ODN stimulation. The images were obtained by confocal microscopy. Scale bar indicates 10 μm. Experiments were repeated three times, and representative images are shown. Source data are available online for this figure. Yasunori Shintani et al. EMBO Rep. 2014;embr © as stated in the article, figure or figure legend

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