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Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier.

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Presentation on theme: "Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier."— Presentation transcript:

1 Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier Muñoz, Gabriel Capellà, Carlos A. González, Antonio Agudo, Núria Sala  The Journal of Molecular Diagnostics  Volume 12, Issue 3, Pages (May 2010) DOI: /jmoldx Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 GSTM1 and GSTT1 null polymorphism genotyping by real-time PCR and melting curve analysis in the presence of SYBR Green. Tm melting peaks of the GSTT1, GSTM1, and the BCL2 amplification control gene in: The Light Cycler 2.0 (A), and The Light Cycler 480 (B). Red line corresponds to GSTM1 and GSTT1 present genotype, blue to GSTM1 null and GSTT1 present genotype, brown to GSTM1 present and GSTT1 null genotype and green to GSTM1 and GSTT1 null genotype. “+” corresponds to the heterozygous or homozygous genotype for the gene presence. “−” corresponds to the homozygous null genotype. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions


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