1. Simultaneous Genotyping of rs and rs Variants Near the IL28B Locus Associated with HCV Clearance and Treatment Response 
Roberta Melis, Christiane Fauron, Gwendolyn McMillin, Elaine Lyon, Brian Shirts, Lindsey M. Hubley, Patricia R. Slev 
The Journal of Molecular Diagnostics 
Volume 13, Issue 4, Pages (July 2011)
DOI: /j.jmoldx
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Multiplexed FRET rs and rs genotyping results. Melting analysis curves of multiplexed, asymmetric, real-time PCR and dual-color FRET probes of three individuals. A: rs homozygous for TT (blue) with Tm at 57.6°C, homozygous for CC (red) with Tm at 66.8°C, and heterozygous for TC (green) analyzed with the F2 channel. B: rs homozygous for TT (blue) with Tm at 61.9°C, homozygous for GG (red) with Tm at 69.3°C, and heterozygous for TG (green) analyzed with the F3 channel. The gray line indicates negative (no template) control.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Multiplexed SNE genotyping results of rs and rs Electropherograms of the SNE reaction products are shown for three different individuals: rs heterozygous for CT; rs heterozygous for GT (A); rs homozygous for CC; rs homozygous for TT (B); and rs homozygous TT; rs homozygous for GG (C).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
Histogram of frequencies of rs /rs compound genotypes observed across ethnic populations. Genotype frequencies were obtained for whites (CAU; n = 125), Asians (AS; n = 71), Middle Easterners (ME; n = 10), Hispanics (HIS; n = 118), and African Americans (AA; n = 119). Six different rs /rs compound genotypes are shown on the x axis. The rs C and the rs T favorable alleles associated with efficacy in virus clearance and treatment outcome.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
Frequency comparisons of rs CC and rs TT favorable genotypes, associated with efficacy in HCV virus clearance and treatment outcome; and rs CT + TT and rs TG + GG risk genotypes across different ethnic groups. CAU indicates white; AS, Asian; ME, Middle Eastern; HIS, Hispanic; and AA, African American.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

6. Figure 5
The distribution of rs /rs haplotypes in whites (CAU), Asians (AS), Middle Easterners (ME), Hispanics (HIS), and African Americans (AA). Haplotype frequencies were obtained from the analysis of 886 chromosomes. Frequencies of C-T, T-T, and T-G haplotypes are shown on the x axis. The C-G haplotype was not observed. F indicates that a favorable haplotype is associated with efficacy in virus clearance and treatment outcome. The rs C and rs T favorable alleles are represented in bold and gray shaded. I indicates indeterminate haplotype with the presence of one risk allele (rs T) and the presence of the rs T favorable allele. R indicates that the risk haplotype is associated with the presence of two risk alleles (rs T and rs G).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions