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Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine.

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Presentation on theme: "Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine."— Presentation transcript:

1 Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine Molecular Diagnostics  Lina Zhang, Guanglin Cui, Zongzhe Li, Haoran Wang, Hu Ding, Dao Wen Wang  The Journal of Molecular Diagnostics  Volume 15, Issue 5, Pages (September 2013) DOI: /j.jmoldx Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Representative genotype results of rs by each method. A and E: Sanger sequencing. Red and black boxes indicate rs (G>A) allele and unexpected heterozygous variant, respectively. B: TaqMan allelic discrimination assay. The three groups indicate three different genotypes of rs (red, wild type; green, heterozygous; and blue, homozygous variant; black, negative controls). C and D: HRMA normalized melting curves and difference plot. Samples were classified into four clusters indicating three different genotypes of rs (red, wild type; green, heterozygous; blue, homozygous variant) and unexpected heterozygous variant (gray curves). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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