1. (x 10-3)
(x 10-4)
GST
P450 3 12 2 8 1 4 WT
mock WT
#15 WT
mock WT
#15
BTH
BTH
(x 10-4)
(x 10-2) 12 20
PR-1a
PR-1b 15 8 10 4 5 WT
mock WT
#15 WT
mock WT
#15
BTH
BTH
(x 10-2) 5
1.5
lipoxygenase
Rubq1 4 1 3 2
0.5 1 WT
mock WT
#15 WT
mock WT
#15
BTH
BTH
Figure S1. Quantitative RT-PCR analysis of the dependence of the BTH-responsive genes on WRKY45.
T2 homozygotes of WRKY45-kd rice plants (#15) and wild-type plants were treated with 0.5 mM BTH, and RNAs were extracted 48 h after treatment. The expression of selected BTH-responsive genes was analyzed by quantitative RT-PCR as in Figure 5. The expression level of Rubq1 is relative to that in mock-treated wild-type plants. The experiments were done twice with similar results.