1. Supplemental Digital Content 4
Fold change in expression
0.3
0.6
0.9
1.2
Non-
transfected
Control siRNA
TF siRNA
Figure 4. Quantitative detection of a-Gal gene expression in NICC. Quantitative detection of a-Gal gene expression was performed by real-time PCR using RNA samples isolated from NICC before and after transfection with TF- or control-siRNA. a-Gal gene expression is shown as relative expression compared to the internal control gene -actin. Data represent the mean ± SEM of three independent experiments.