1 CONFIDENTIAL ULTRAXIDE - FIGHT H5N1! Avian Influenza ( BIRD FLU) Bird flu is an infection caused by avian (bird) influenza (flu) viruses. bird flu is very contagious among birds and can make some domesticated birds, including chickens, ducks, and turkeys, very sick and kill them. Influenza A (H5N1) virus – also called “H5N1 virus” – is an influenza A virus subtype that occurs mainly in birds. Recent finding also states that similar virus has been isolated in swineHuman symptomsSymptoms of bird flu in humans have ranged from typical flu-like symptoms (fever, cough, sore throat and muscle aches) to eye infections, pneumonia, severe respiratory diseases (such as acute respiratory distress), and other severe and life-threatening complications.Human Influenza riskDuring an outbreak of bird flu among poultry (domesticated chicken, ducks, turkeys), there is a possible risk to people who have contact with infected birds or surfaces that have been contaminated with excretions from infected birds. The current outbreak of avian influenza A (H5N1) among poultry in Asia and Europe is an example of a bird flu outbreak that has caused human infections and deaths. In such situations, people should avoid contact with infected birds or contaminated surfaces, and should be careful when handling and cooking poultry..Bird flu preventionThere currently is no commercially available vaccine to protect humans against the H5N1 virus that is being seen in Asia and Europe . Although vaccine development is in progress, the best method to fight the infection and its spread would be to prevent the virus from thriving in the environment, thru a solid disinfection and biosecurity measure.CONFIDENTIAL
2 CONFIDENTIAL ULTRAXIDE - FIGHT H5N1! What is Ultraxide? ULTRAXIDE is a high performance disinfectant formulated for use in poultry hatcheries, livestock buildings and for disinfecting equipment. A potent disinfectant, it provides control over pathogenic bacteria , fungi and viruses such as Bird flu virus , Gumboro Virus, New Castle Disease and Swine Vesicular Disease.CONFIDENTIAL
3 CONFIDENTIAL ULTRAXIDE - FIGHT H5N1! Advantages of Ultraxide Broad Spectrum – effective against non-enveloped and enveloped viruses. Independent tests proves effective against all 17 virus families affecting man and animals. Excellent activity against bacteria, mycoplsma moulds, fungi and spores.Non-corrosive, non-tainting, no environmental residue problems, exceptionally low toxicity.Rapid action timeCan be applied to surfaces and equipment to clean and disinfect in one-step operation.An excellent and safe water sterillant.May be used as an aerial spray.Suitable use on all types of farms, in hatcheries and processing plants.Pleasant smell and residual activity up to 1 week.CONFIDENTIAL
4 Mode of Action of Ultraxide GLUTARALDEHYDE :Glutaraldehyde has the property to bind itself to the proteins through their amine groups.It has a very strong virucidal activity obtained by the cross-linking of the proteins of the naked (or hydrophilic) viruses ’ capsid.It also combines with the nucleic acids contained in viruses DNA/RNA). Bactericidal and fungicidal activities are due to the combination of glutaraldehyde with the proteinic parts of metabolic enzymes, and with the amino-acids.Being strictly hydrophilic, glutaraldehyde cannot cross the cell's lipid membrane nor the coating of lipophilic viruses.Therefore it can only act outside these micro-organisms and has consequently a rather slow action.Ultraxide formula allows glutaraldehyde to kill germs from the inside due to synergistic blend with highly tensio-active Quaternary Ammonium Chloride ( QAC).Quaternary Ammonium ChlorideQAC's are cationic tensio-active agents. At low concentration they have cidal properties on a wide spectrum of micro-organisms (Gram+ & Gram- bacteria,fungi, viruses).Their major site of action is the cell membrane, where they create a dissolution of phospholipids (cell membranes) and cause changes in permeability that allow the escape of cell constituents and cause cell disorientation.These changes in permeability allow glutaraldehyde to penetrate inside the microorganisms.CONFIDENTIAL
5 Virucidal activity of Ultraxide Virus Family Effective DilutionAdenoviridae :100Herpesviridae :600Iridoviridae :100Papovaviridae :200Parvoviridae :50Poxviridae :300Bunyaviridae :400Coronaviridae :280Birnaviridae 1:150Virus Family Effective DilutionOrthomyxoviridae(Bird flu) 1:320Paramyxoviridae :280Picornaviridae :200Reoviridae :250Retroviridae :200Rhabdoviridae :200Togaviridae :350Hepadnaviridae :100CONFIDENTIAL
6 BIRD FLU CONTROL PROGRAMME A . POULTRY OPERATION:All-in and all-out must be practiced to avoid recontamination of the new generation of flock.Clean all premises. Remove all the residues, remnants of the former batch of flock since this decrease the effectivity of the disinfectant.All removable equipments should be detached, cleaned and disinfected.Wash down roads, sheds, sewerage.Power wash shafts, vents, and other surfaces with water.Practice terminal disinfection with Ultraxide® 1 : 100. (eg. 25 liters of Ultraxide® mixed with 2,500 liters for 75,000 sq ft. of surface area which is equivalent to 20,000 sq ft. broiler house.)Newly washed equipment are replaced inside.Fumigate the houses using foggers with Ultraxide® 1 : 5.Put Ultraxide® in foot dips at 1 : 100 situated outside the entrance doors.Do not allow entry of personnel after the cleaning and sanitation procedures.CONFIDENTIAL
7 CONFIDENTIAL I. POULTRY HOUSE / BUILDING: II. EQUIPMENT DISINFECTANT Power wash shafts, vents, and other surfaces efficiently with waterNewly washed equipment are replaced inside.CONFIDENTIALII. EQUIPMENT DISINFECTANTClean equipments.Dip in Ultraxide at 1 : 200.Return equipment to the poultry house to avoid recontamination of pathogens in the environment.Flush out drinker lines with 1 : 3,000 at the end of every cycle of broiler and layer production.
8 CONFIDENTIAL III. HATCHERY DISINFECTION Wash down tray deck equipment after egg sorting.Drying time minutes.Fumigate room with Ultraxide 1 : 5.For egg storage areas, wash floor areas daily.Fog daily with Ultraxide 1 : 5 the egg storage areas.Trolleys should also be washed, be left in the room for fogging.Buggies and trays must be washed with Ultraxide at 1 : 200.Remove all debris from the hatchery.CONFIDENTIAL
9 CONFIDENTIAL IV. SETTER ROOMS VACCINATION AND HOLDING ROOMS After candling the eggs, surfaces, floors and walls should be washed daily. The surfaces should be dried.Apply Ultraxide 1 : 200 on surfaces. Then, fumigate at the end of the day at 1 : 5.Setter trays should be taken to wash area to be cleaned and soaked with Ultraxide at 1 : 400.CONFIDENTIALVACCINATION AND HOLDING ROOMSWash all surfaces including work tops, sexing tables, chairs. Leave for 30 minutes to dry.Ultraxide at 1 : 150Fog Room- at the end of the day with Ultraxide at 1 : 5.
10 CONFIDENTIAL VI. CORRIDORS VII. SPECIFIC USES Ultraxide Remove soil or organic materials before disinfection. Spray with Ultraxide 1 : 400.CONFIDENTIALVII. SPECIFIC USES UltraxideKills influenza virus upon contact after it is shed in the environmentInactivates Infectious Bursal Disease at 1 : 150Newcastle Disease at 1 : 200Minimize Coccidial PopulationTerminal Disinfection
11 CONFIDENTIAL Packing Storage Ultraxide is packed in 1,5,20,200 litre containers.StorageStore in a cool, dry place. Storage temperature should not be more than 50oC.CONFIDENTIAL
12 CONFIDENTIAL Dilution Chart for Ultraxide Items Dilution Rate Hatching Room 1:150Livestock Building 1:150Wash Room 1:150Equipment 1:150Egg store 1:320Vehicles 1:400Drinking water 1:3000General Purposedisinfection 1:150 ~1:400CONFIDENTIAL