1. Phosphorylation of RelA Ser311 in vivo.
Phosphorylation of RelA Ser311 in vivo. (A) Control EFs or stably expressing HA‐tagged wild‐type or S311A mutant RelA were incubated with TNF‐α for different times, after which the ectopically expressed RelA was immunoprecipitated with the anti‐HA antibody and Ser311 phosphorylation was determined by immunoblotting with the anti‐phospho‐RelA(Ser311) antibody (upper panel). The lower panel shows a parallel immunoblot with the anti‐HA antibody. (B) The immunoprecipitates from the cells that had been treated with TNF‐α for 30 min were immunoblotted with the anti‐phospho‐RelA(Ser311) antibody in the absence or presence of phospho‐ or dephospho‐peptide. (C) Wild‐type or ζPKC‐deficient EFs were stimulated with TNF‐α for different times, after which endogenous RelA was immunoprecipitated and Ser311 phosphorylation was determined by immunoblotting as above.
Angeles Duran et al. EMBO J. 2003;22:
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