1. Published in Thyroid. January 2014, 24(1): 88-168. DOI: 10. 1089/thy
Published in Thyroid. January 2014, 24(1): DOI: /thy © Mary Ann Liebert, Inc.
FIG. 1.  Microscopic structure of the mouse thyroid. (A) Hematoxylin and eosin (H&E) staining. (B) Periodic acid Schiff (PAS) staining. Mice were euthanized, and the thyroids dissected, fixed in buffered formalin, and embedded in paraffin. Thyroid sections (5 μm) were mounted on glass slides, de-paraffinated, and hydrated. For histological analysis, sections were stained with H&E, following a standard protocol. Glycoproteins were detected using PAS staining. Sections were stained with 0.5% periodic acid for 30 minutes and with Schiff's reagent for 20 minutes and then rinsed in running tap water for 5 minutes. Nuclei were counterstained with hematoxylin for 3 minutes. Sections were rinsed in running tap water, dehydrated, cleared, and mounted. Reproduced with permission from Senou et al. (20).