1. From: IGF-1 Regulates the Extracellular Level of Active MMP-2 and Promotes Müller Glial Cell Motility
Invest. Ophthalmol. Vis. Sci ;56(11): doi: /iovs
Figure Legend:
IGF-1 induces IGF-1R autophosphorylation and downstream activation of intracellular signaling pathways in MIO-M1 cells. (A) Western blot analysis of phosphorylated IGF-1R (p-IGF-1R) in MIO-M1 cells cultured in the presence of 10 nM IGF-1 at indicated times. IGF-1R is shown as a loading control. (B) Representative Western blot assay of phosphorylated AKT (p-AKT) in MIO-M1 cells cultured in the presence of 10 nM IGF-1 at indicated times. AKT 1/2/3 is shown as a loading control. The bars represent the densitometric analysis of the immunoreactive protein expressed as relative intensity calculated from the densitometric intensity of p-AKT signal normalized to AKT 1/2/3. Bars represent the mean ± SD of three independent experiments. The asterisks show statistical differences with respect to control (0 minutes) for P < 0.01 (**) and P < (***), respectively. (C) Representative Western blot assay of phosphorylated ERK 1/2 (p-ERK 1/2) in MIO-M1 cells cultured in the presence of 10 nM IGF-1 at indicated times. ERK 1/2 is shown as a loading control. The bars represent the densitometric analysis of the immunoreactive protein expressed as relative intensity calculated from the densitometric intensity of p-ERK 1/2 signal normalized to ERK 1/2. Bars represent the mean ± SD of three independent experiments. ns, nonsignificant. The asterisks show statistical differences with respect to control (0 minutes) for P < (***). (D) Western blot assay of lysates from cells preincubated (or not) with the αIR3 antibody in the presence or absence of 10 nM IGF-1, showing p-IGF-1R, p-AKT, and p-ERK 1/2 levels. IGF-1R is shown as a loading control.
Date of download: 11/22/2017
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