1. From: Preservation of Retina Ganglion Cell Function by Morphine in a Chronic Ocular-Hypertensive Rat Model
Invest. Ophthalmol. Vis. Sci ;53(7): doi: /iovs
Figure Legend:
Inhibition of TNF-α expression in the presence of morphine (1 mg/kg; IP) 3 days post injury. Rats were divided into two groups: ocular-hypertensive group (n = 4); and morphine-treated ocular-hypertensive group (n = 4). Three days post hypertonic-saline injection into the limbal veins, animals were euthanized and retina extracts (15 μg retina protein) were analyzed by Western blotting using selective anti-TNF-α primary antibodies. The signal was captured using appropriate secondary antibodies (HRP-conjugated; dilution 1:3000), enhanced chemiluminescent reagent, and the Biorad Versadoc imaging system. Data shown are representative of four, independent experiments. Data are expressed as mean ± SE. *P < 0.05; n = 4.
Date of download: 10/21/2017
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