1. Local Insulin-Zinc Injection Accelerates Skin Donor Site Wound Healing
Xiao-jun Zhang, M.D., Xiaowu Wu, M.D., Steven E. Wolf, M.D., Hal K. Hawkins, M.D., Ph.D., David L. Chinkes, Ph.D., Robert R. Wolfe, Ph.D. 
Journal of Surgical Research 
Volume 142, Issue 1, Pages (September 2007)
DOI: /j.jss
Copyright © 2007 Elsevier Inc. Terms and Conditions

2. FIG. 1
(A) Effect of local insulin injection on arterial blood glucose concentration. Local injection of 0.25 units of long-acting insulin caused moderate decreases in blood glucose concentration during the first 3 h and returned to the basal value thereafter. In contrast, local injection of 1.0 unit of insulin caused severe hypoglycemia. (B) Injection of 0.25 units of long acting insulin in another two rabbits caused reproducible changes in arterial glucose concentration. Thus, the dose of 0.25 units was selected for local injection.
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
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3. FIG. 2
Insulin concentration in the skin wound fluid after local insulin of 0.25 units of long-acting insulin. The concentration of insulin in the wound remained ≥50 μU/mL for at least 24 h. Thus, the injection frequency was selected as every other day.
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
Copyright © 2007 Elsevier Inc. Terms and Conditions

4. FIG. 3
(A) the appearance of the donor wound created by the electric dermatome at in. There was dermal tissue on the wound surface, characteristic of a partial thickness wound. (B) light micrograph (H&E-stained) of the skin donor site wound sampled immediately after wounding shows deep fascia, panniculus carnosus muscle, and fibrous connective tissue. The epidermis is absent, and no follicles or other epidermal appendages are seen, except for the tips of a few follicles (arrows point at residuals of hair follicles). These findings are typical of a partial thickness wound. (Color version of figure is available online.)
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
Copyright © 2007 Elsevier Inc. Terms and Conditions

5. FIG. 4
Daily chow intake in rabbits. The amounts of chow consumption after injury were not significantly different (P > 0.05) between the two groups except on day 5 the insulin group consumed less (P < 0.05) chow than the control group.
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
Copyright © 2007 Elsevier Inc. Terms and Conditions

6. FIG. 5
(A) A representative wound in the control group. On day 9 after injury, the wound was not healed. There was a large open wound. This wound was fully healed on day 20. (B) A representative wound in the local insulin group. The wound was fully healed on post-injury day 9. (Color version of figure is available online.)
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
Copyright © 2007 Elsevier Inc. Terms and Conditions

7. FIG. 6
Correlation (r = −0.66, P = 0.02) between epidermal growth rate and days for wound healing. The faster the epidermal growth was, the less the number of days required for wound healing.
Journal of Surgical Research  , 90-96DOI: ( /j.jss )
Copyright © 2007 Elsevier Inc. Terms and Conditions