Download presentation
Presentation is loading. Please wait.
Published byAntal Gál Modified over 6 years ago
1
Limb ischemia–reperfusion differentially affects the periosteal and synovial microcirculation
Petra Hartmann, MD, PhD, Gábor Erős, MD, PhD, Renáta Varga, MD, PhD, József Kaszaki, PhD, Dénes Garab, István Németh, MD, Zsolt Rázga, PhD, Mihály Boros, MD, PhD, Andrea Szabó, MD, PhD Journal of Surgical Research Volume 178, Issue 1, Pages (November 2012) DOI: /j.jss Copyright © 2012 Elsevier Inc. Terms and Conditions
2
Fig. 1 RBCV values measured in capillaries of periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K-injected limbs served as positive controls, and contralateral limbs were injected with saline (Saline). Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. $P < 0.05 versus P-Sham within control groups; ∗P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
3
Fig. 2 Rolling leukocytes in postcapillary venules of periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K-injected limbs served as positive controls, and contralateral limbs were injected with saline (Saline). Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. *P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
4
Fig. 3 Sticking leukocytes in postcapillary venules of periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K-injected limbs served as positive controls, and contralateral limbs were injected with saline (Saline). Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. ∗P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
5
Fig. 4 XOR activity measured in periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K- injected limbs served as positive control, and contralateral limbs were injected with saline (Saline). Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. ∗P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
6
Fig. 5 MPO measured in periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K-injected limbs served as positive controls, and contralateral limbs were injected with saline (Saline). Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. ∗P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
7
Fig. 6 (A) ICAM-1 expression in periosteum (P) and synovial membrane (SY) in sham-operated (Sham) limbs and limbs subjected to total limb IR injury. C/K-injected limbs served as positive controls, and contralateral limbs were injected with saline (Saline). Representative micrographs show positive reactions for ICAM-1 in (B) IR-challenged periosteum and (C) SY in response to C/K injection. Bar denotes 50 μm. Data are presented as mean ± SEM; 2-way ANOVA was followed by pairwise comparisons of the mean using the Holm-Sidak test. ∗P < 0.05 versus corresponding control; ×P < 0.05 versus P + IR; #P < 0.05 versus SY + IR. (Color version of figure is available online.) Journal of Surgical Research , DOI: ( /j.jss ) Copyright © 2012 Elsevier Inc. Terms and Conditions
Similar presentations
© 2024 SlidePlayer.com Inc.
All rights reserved.