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Visualization of RNA–protein interactions in living cells: FMRP and IMP1 interact on mRNAs by Oliver Rackham, and Chris M Brown EMBO J. Volume 23(16):

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Presentation on theme: "Visualization of RNA–protein interactions in living cells: FMRP and IMP1 interact on mRNAs by Oliver Rackham, and Chris M Brown EMBO J. Volume 23(16):"— Presentation transcript:

1 Visualization of RNA–protein interactions in living cells: FMRP and IMP1 interact on mRNAs by Oliver Rackham, and Chris M Brown EMBO J. Volume 23(16):3346-3355 August 18, 2004 ©2004 by European Molecular Biology Organization

2 A TriFC method to study RNA–protein interactions in living cells. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

3 Detection of specific IMP1–zipcode interactions in living cells. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

4 Detection of specific IRP1–IRE interactions in living cells. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

5 Detection of specific FMRP–RNA interactions in living cells. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

6 IMP1, FMRP, hStau and PTB associate with multiple mRNA sequences in vivo. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

7 IMP1 and FMRP associate with zipcode and FMR1 3′UTR RNA sequences. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

8 FMRP and IMP1 interact in the absence of reporter mRNA. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

9 FMRP and IMP1 associate via protein–protein interactions. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

10 Tethered IMP1 recruits FMRP to an mRNA, and tethered FMRP can recruit IMP1 to an mRNA. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

11 Model of the mRNA localization process involving IMP1 and FMRP. The localization process begins in the nucleus, with IMP1 and FMRP associating with the RNA at the transcription site. Oliver Rackham, and Chris M Brown EMBO J. 2004;23:3346-3355 ©2004 by European Molecular Biology Organization

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