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Altered nucleotide misinsertion fidelity associated with polι ‐ dependent replication at the end of a DNA template by Ekaterina G. Frank, Agnès Tissier,

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Presentation on theme: "Altered nucleotide misinsertion fidelity associated with polι ‐ dependent replication at the end of a DNA template by Ekaterina G. Frank, Agnès Tissier,"— Presentation transcript:

1 Altered nucleotide misinsertion fidelity associated with polι ‐ dependent replication at the end of a DNA template by Ekaterina G. Frank, Agnès Tissier, John P. McDonald, Vesna Rapić ‐ Otrin, Xianmin Zeng, Patricia J. Gearhart, and Roger Woodgate EMBO J. Volume 20(11):2914-2922 June 1, 2001 ©2001 by European Molecular Biology Organization

2 Nucleotide sequence and structure of DNA templates used in the replication assays described in Figure 2. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization

3 (A) Comparison of polι's ability to replicate primed single ‐ stranded DNA and gapped templates. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization

4 Ability of polι to incorporate nucleotides at the end of a DNA template. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization

5 (A) Nucleotide sequence of the primer and templates utilized to measure polι ‐ dependent primer extension. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization

6 (A) Nucleotide sequence and structure of DNA templates used in replication assays to measure polι ‐ dependent extension from a correctly paired 22mer and a 22mer with two terminal mismatches. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization

7 Northern blot analysis of POLI mRNA in adult tissues. Ekaterina G. Frank et al. EMBO J. 2001;20:2914-2922 ©2001 by European Molecular Biology Organization


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