1. Lab Safety NANO 250 April 14 th, 2015

2. Who is responsible for safety? Safety Laser training Radiation training Biohazard Training X-ray training Specialization Instrument Cell culture Animal studies Clean room Occupational Safety & Health Administration Organization’s H & S

3. PPE

4. NFPA Diamond Scale?0 to 4 Health Flammability Special W Ox Often yield oxygen (flammable)

5. International Symbols Explosive (E) Highly Flammable (F) Extremely Flammable (F+) Toxic (T) Very Toxic (T+) Harmful (Xn) Irritant (Xi) Corrosive (C) Dangerous for Environment (N) Explosive Oxidizing (O) Highly Flammable (F) Extremely Flammable (F+) Highly Flammable (F) Extremely Flammable (F+) Toxic (T) Very Toxic (T+) Toxic (T) Very Toxic (T+) Harmful (Xn) Irritant (Xi) Harmful (Xn) Irritant (Xi) Corrosive (C) Corrosive (C) Dangerous for Environment (N) Dangerous for Environment (N)

6. Chemical Storage Acids, Bases, flammables, solvents Pyrophoric chemicals (ignite spontaneously) Light-sensitive chemicals Water-sensitive chemicals Secondary Containment

7. Waste Storage & Disposal 1.Label (name, date, contents) 2.Segregate 3.Proper storage 4.Cap! Loosely if afraid of explosion 5.Don’t accumulate!

8. Human-caused Contamination With normal activity, a human generates five to ten million particles per minute! 8 Gowning! Gloves Safety Glasses Hair Net Lab Coat Shoe Covers PUT ON TAKE OFF http://www.cintas.com/CleanroomResources/popups/gowning/index.html

9. Clean Room Do’s and Don’ts Don’t: – Touch your face or skin with gloves – Lean on equipment – Wear rings / bracelets (can get damaged!) – Use paper, pencils, or markers that leave dust Do: – Change gloves before handling a wafer – Wipe down wafer handling areas with isopropanol 9

10. http://www.coastwidelabs.com/Technical%20Ar ticles/Cleaning%20the%20Cleanroom.htm