1. University Vienna Dept. Molecular Systems Biology PPMetabolomics Ass. Student: Mara Vallianou Theoretical Introduction: 1x Experimental (Wienkoop) 2 x Analytics (Fragner/Wienkoop) 2 x Systems Biology (Weckwerth) 1 x Statistics (Bachmann) Practical Introduction: Lena Stefanie Florian Nastassja Lena Fragner Stefanie Wienkoop PP Leitung Wolfram WeckwerthGert Bachmann Team in alphabetical order Ass. Students: Florian Demel + Nastassja Holeschofsky

2. University Vienna Dept. Molecular Systems Biology Stefanie Wienkoop - PPMetabolomics Schedule Mometabolite extraction, theory Tu derivatization, measurements, software We statistics, data analysis Thdata mining, theory Fr data mining, theory

3. University Vienna Dept. Molecular Systems Biology Stefanie Wienkoop - PPMetabolomics Material – Extraction – GC-MS: 3 groups á 4 persons Experiment - Data mining: 4 Groups á 3 persons Material – Extraction – GC-MS: Group 1) Medicago truncatula: leaf cold stress/control Group 2) Lepidium cartilagineum : leaf salt stress/control Group 3) Arabidopsis thaliana: leaf cold stress/control

4. University Vienna Dept. Molecular Systems Biology Stefanie Wienkoop - PPMetabolomics Experiments - Data mining Group 1) Medicago truncatula: leaf cold stress/control Group 2) Lepidium cartilagineum : leaf salt stress/control Group 3) Arabidopsis thaliana: leaf cold stress/control Group 4) Leaves Ara/Medic/Lep

5. University Vienna Dept. Molecular Systems Biology What do we expect from you? 1)Excellent collaboration 2)Final presentation of your results in english: a) Protocol (per group) b) Oral presentation (per group) (date according to prior agreement) Stefanie Wienkoop – Plant Systems Interaction Stefanie Wienkoop - PPMetabolomics

6. University Vienna Dept. Molecular Systems Biology MS-Techniques 1)Metabolite Extraction 2)GC-Triple quadrupole MS (Metabolite profiling and quantitative analysis) 3) Data Mining – Statistical Analyses Tools (Multivariate) Stefanie Wienkoop – Plant Systems Interaction Stefanie Wienkoop - PPMetabolomics

7. University Vienna Dept. Molecular Systems Biology PPMetabolomics Stefanie Wienkoop – Plant Systems Interaction ecology learning

8. University Vienna Dept. Molecular Systems Biology PPMetabolomics Stefanie Wienkoop – Plant Systems Interaction Login: User: pdfuser Password: pdfpass

9. University Vienna Dept. Molecular Systems Biology PP Metabolomics Lab work

10. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab Safety Chemicals Chloroform: http://www.sigmaaldrich.com/MSDS/MSDS/

11. University Vienna PP Metabolomics – Lab Safety Chemicals Chloroform: Wirkungscharakter: Narkotisch, sensibilisierend für Katecholamine am Herzen! Lebertoxisch; kardiotoxisch! Im Tierversuch karzinogen. Chronisch hepatotoxisch; degenerative ZNS-Veränderungen bei chronischer Zufuhr (BOMSKI et al., 1967). Letaldosis: bei peroraler Zufuhr von 10 ml Chloroform tritt der Tod durch Lähmung des ZNS ein; Atemdepression (KAEMPE et al., 1980). http://toxcenter.org/stoff-infos/c/chloroform.pdf Dept. Molecular Systems Biology

12. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab Safety Chemicals Methanol: http://www.sigmaaldrich.com/MSDS/MSDS/

13. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab Safety Chemicals

14. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab Safety Chemicals Methanol: http://toxcenter.org/stoff-infos/c/chloroform.pdf

15. Personal Safety Equipment Contact lenses

16. University Vienna Dept. Molecular Systems Biology

17. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab work Ernte Homogenisieren Lab Work

18. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab work Extraction

19. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab work Derivatisierung

20. University Vienna Dept. Molecular Systems Biology PP Metabolomics – Lab work Viel Spaß und gutes Gelingen!!!