1. Equipment for Small Rodents Corporate Marketing Dept.

2. 1.How to configure the Ideal Housing Solution 2.Housing Guidelines 3.Thermal Properties 4.Chemical Resistance 5.How To Wash Plastic Cages 6.How To Sterilize Cages In Autoclave Table of Contents

3. How to configure the Ideal Housing Solution First Step: Select the Cage Body Second Step: Select the Lid Third Step: Select the Bottle & Bottle Cap Fourth Step: Select the Label Holder Fifth Step: Select the Accessories Sixth Step: Select the Rack 1 6 3 5 4 2

4. EU Housing Guidelines SpeciesBody Weight (g) Minimum Enclosure Size (cm 2 ) Floor Area per Animal (cm 2 ) Minimum Enclosure Height (cm) Mice< 20 20 – 25 25 – 30 > 30 330 60 70 80 100 12 Rats< 200 200 – 300 300 – 400 400 – 600 > 600 800 1500 200 250 350 450 600 18 Hamsters< 60 60 – 100 > 100 800 150 200 250 14 Guinea Pigs < 200 200 – 300 300 – 450 450 – 700 > 700 1800 2500 200 350 500 700 900 23 Number of Animals per Cage Weight of Animals (g) Number of Rats per Cage Number of Mice per Cage < 200 200 - 300 300 - 400 > 600 400 – 600 < 20 20 - 25 25 - 30 > 30 Number of Guinea Pigs per Cage Weight of Animal (g) Number of Animals per Cage < 200 200 - 300 300 - 450

5. UK (Home Office) Housing Guidelines SpeciesWeight of Animal (g) Minimum Floor Area per Animal when Housed in Groups (cm 2 ) Minimum Floor Area per Animal when Housed Singly (cm 2 ) Minimum Height (cm) Mice< 20 20 – 25 25 – 30 > 30 330 60 70 80 100 12 Rats< 200 200 – 300 300 – 400 400 – 600 > 600 800 1500 200 250 350 450 600 18 Hamsters< 60 60 – 100 > 100 800 150 200 250 14 Guinea Pigs < 200 200 – 300 300 – 450 450 – 700 > 700 1800 2500 200 350 500 700 900 23 Number of Animals per Cage Weight of Animals (g) Number of Rats per Cage Number of Mice per Cage < 200 200 - 300 300 - 400 > 600 400 – 600 < 20 20 - 25 25 - 30 > 30 Number of Guinea Pigs per Cage Weight of Animal (g) Number of Animals per Cage < 200 200 - 300 300 - 450

6. USA Housing Guidelines SpeciesWeight (g) Floor Area per Animal (cm 2 ) Minimum Height (cm) Mice< 10 10 – 15 15 – 25 > 25 38 51 77 > 96 12 Rats< 100 100 – 200 200 – 300 300 – 400 400 – 500 > 500 109 148 187 258 387 > 451 17 Hamsters< 60 60 – 80 80 – 100 > 100 64 83 103 > 122 15 Guinea Pigs < 350 > 350 387 > 651 17 Number of Mice per Cage Number of Animals per Cage Weight of Animals (g) <10 10-15 15-25 >25 Number of Animals per Cage Number of Rats per Cage Weight of Animals (g) <100 100-200 200-300 300-400 400-500 >500 Number of Guinea Pigs per Cage Number of Animals per Cage Weight of Animals (g) <350 >350

7. Cage Body Selection 1291H Eurostandard Type III H 425x266x185mm – Floor Area 800cm 2 2154F 480x265x210mm – Floor Area 940cm 2 2150E 355x235x190mm – Floor Area 580cm 2 Rats/Hamsters 2000P 610x435x215mm – Floor Area 2065cm 2 1354G Eurostandard Type IV 595x380x200mm – Floor Area 1820cm 2 1500U Eurostandard Type IV S 480x375x210mm – Floor Area 1500cm 2 Guinea Pigs * As well suitable for Rats Polycarbonate (PC) H-Temp Polysulfone (PSU) U-Temp Polyetherimide (PEI) Plastic Selection 1144B 332x150x130mm – Floor Area 335cm 2 1145T 369x156x132mm – Floor Area 435cm 2 1264C Eurostandard Type II 267x207x140mm – Floor Area 370cm 2 1284L Eurostandard Type II L 365x207x140mm – Floor Area 530cm 2 1290D Eurostandard Type III * 425x266x155mm – Floor Area 820cm 2 Mice 3 years warranty 5 years warranty 1 year warranty

8. Lid Selection Wire Lid Removeable Divider -116 Inner Fitting Lid U-Shaped Feed Hopper, Hinged Divider and Swing-Clip Lock -114 Inner Fitting Lid U-Shaped Feed Hopper, Hinged Divider and Front-Clip Closure Difference -012 Outer Fitting Lid U-Shaped Feed Hopper without Hinged Divider -025 Outer Fitting Lid Flad Lid without Feed Hopper Optional Feed Hopper Divider Raised Wire Lid Drop-in Hopper -123 U-Shaped Feed Hopper, Hinged Divider and Front-Clip Closure -120 U-Shaped Feed Hopper, Hinged Divider and Swing-Clip Lock -962/-965 (For Guinea Pigs) -950/-952 (For Rats) -119 / -224 Drop-In Hopper and Swing Clip Lock

9. Bottle & Cap Selection Bottles with Silicone Ring Bottle Selection Silicone Ring (Detail) ACBT0152 150ml ACBT0251 250ml ACBT0502 500ml ACBT0402 400ml ACBT0702 700ml ACBT0752 750ml ACBT0262 260ml ACBT1000 1000ml * Bottles 260ml, 400ml and 700ml are as well available in other kinds of plastic Cap Selection ACCPLC01 Cap Remover ACCP2521 SS Standard Cap with Ø 1,8 mm Hole ACCP2511 SS Cap with Ø 1,0 mm Hole ACCPS50 SS Cap with Balls ACCP6521 SS Cap for small, weak or post surgery Animals ACCP2522 Nylon Cap

10. Label Holder Selection With SpringWith Hook Vertical Horizontal * Label Holders are available for each Cage Body

11. Accessories Difference -400SU Cage with Filter Top without Nylon Lock Latch Efficient gaseous interchangeNylon Lock Latch PSU H-Temp FramePolyester Filter Sheet and SS Retainer Filter Top Powder Feeder -927 -922 Two-Part Design Consisting of Lower Tray/Hopper -929 “J”-Design Plastic Bag ACBAG001SR For Autoclaving Cages Raised Bottom Grid -150 AISI 304 SS Wire Mesh

12. Rack Selection Demountable Shelf Style RackWelded Shelf Style Rack Runner Style Rack Polyurethane Coated CastorsNylon Runners Mesh Bottom Cage Double Runner RackStandalone Solution Adjustable Shelf Style Rack

13. Thermal Properties

14. Chemical Resistance

15. How To Wash Plastic Cages An Alkaline Washing Cycle is recommended to remove organic soiling. An Acid Washing Cycle is recommended to remove urine or hard water scaling. Procedure 1.Wash with a detergent specific for plastic materials (see table for chemical resistance of each plastic material) 2.If an alkaline detergent is used, carry out a neutralization cycle 3.Rinse with soft water Safety Notes DO NOT exceed the suggested washing temperature since higher washing temperature could damage the parts DO NOT use rinse aid products IMPORTANT ALKALINE DETERGENTS CAN DAMAGE POLYCARBONATE PARTS (CORROSION AND HYDROLYSIS) IF NOT THOROUGHLY NEUTRALIZED! TO NEUTRALIZE CARRY OUT A SHORT ACID CYCLE AND RINSE WITH SOFT WATER. SHOULD SOME WATER RESIDUES REMAIN ON THE CAGES, ENSURE THAT THE PH IS EITHER NEUTRAL OR SLIGHTLY ACID BEFORE HANDLING.

16. How To Sterilize Plastic Cages In Autoclave It is possible to autoclave fully assembled cages Procedure 1.Place cages in the autoclave It is possible to autoclave up to 10 cage bodies stacked one inside the other Polypropylene cages cannot be stacked during autoclaving It is possible to autoclave fully assembled cage at 121°C/250°F Ensure that all the parts (wire lid, filter top, etc.) are properly positioned in order to avoid damaging the cage DO NOT autoclave plastic label holders on the cages. Remove label holders before autoclaving cages and autoclave them separately at 121°C/250°F 2.Autoclave at the suggested temperature 3.Let the cages cool down on a levelled surface SAFETY NOTES IMPORTANT IF FILTER TOPS ARE FITTED WITH NYLON LOCK CLAMPS, RELEASE THE CLAMPS BEFORE AUTOCLAVING TO AVOID DAMAGING THEM.

17. How To Sterilize Plastic Cages In Autoclave DO NOT exceed the suggested autoclaving temperature DO NOT stack more than 10 cage bodies DO NOT autoclave fully assembled plastic cages with lid series -014/-114 and -123 on, since spring clip may cause cage wall warping Avoid thermal pulsing cycles Before autoclaving ensure that no detergent residues are left on the parts PC and PP parts may gradually weaken or be damaged after repeated autoclaving (warping, clouding, cracking) Clean steam is essential for a proper sterilization. Any residue left by sterilizing system could damage the cages and poison the animals If the parts are autoclaved inside a protective covering (sterile package, cotton, plastic bags), use permeable materials in order to avoid damages to the equipment. Contact Tecniplast for further information