1. StainsStains

2. Negative Stain

3. Cheek Cells Without Stain

4. Cheek Cells with Methylene Blue

5. Differential Stains: Gram Stain Color of Gram + Color of Gram – Primary stain: Crystal violet Purple Mordant: Iodine Purple Decolorizing agent: Alcohol-acetone PurpleColorless Counterstain: Safranin PurplePink

6. Gram Positive and Gram Negative Bacteria

7. Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

8. Capsule Stain

10. Figure 3.12a-c

11. Non Acid-Fast(A) and Acid-Fast (B)

12. Endospore Stain: Ziehl-Neelsen

13. Figure 3.12a-c

14. Endospore Stain

15. Flagella Stain

17. Figure 3.12a-c

18. Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST SPORE STAIN PRIMARY STAINCrystal violet Carbol Fuschia Malachite green MORDANTIodine Heat DECOLORIZERETOH or acetone: Cell is purple ETOH or acetone: Cell is clear Acid alcohol Water COUNTER-STAINSafranin: Cell is purple Safranin: Cell is pink Methylene Blue Safranin: Spores are green Cell is pink