Presentation is loading. Please wait.

Presentation is loading. Please wait.

Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the.

Similar presentations


Presentation on theme: "Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the."— Presentation transcript:

1 Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast and endospore stains Review the structure and function of endospores

2 For Acid Fast Bacteria Use only Carbol fuchsin for AFB!! Acid Alcohol Methylene Blue

3 Modified Kinyoun’s Acid-fast Stain – Cold Method Heat fix smear Primary stain: Carbol fuchsin for AFB, (5 min) Rinse with water Decolorize briefly (~10 drops) with acid alcohol (~ 30 sec) Immediately rinse with water Counterstain: Methylene blue (1 min) Rinse with water

4

5

6

7

8

9

10 Endospore Stain Heat fix smear Primary stain: Malachite green, steam (5 min) Rinse thoroughly with water (~ 30 sec) Counterstain: Safranin (1 min)

11

12

13

14

15


Download ppt "Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the."

Similar presentations


Ads by Google