1. Lentikats Biotechnology in distilleries

2. Content 1. PRINCIPLES Principles of Lentikats Biotechnology
Benefits of Lentikats Biotechnology
Lentikats Biotechnology in distilleries
2. CASE STUDIES
Case study – molasses distillery
Case study – distillery processing sugar solutions
Case study – corn distillery
3. WHAT CAN WE OFFER?
References and experiences, our partners
Contacts

3. Principles of Lentikats Biotechnology

4. What is a Lentikats Biocatalyst?
Unique shape of the matrix and the original principle of immobilisation enable the encapsulation of free enzymes or microorganisms (bacteria, yeasts, fungi) in a stable porous PVA (polyvinylalcohol) carrier. The carrier‘s advantages are:
Excellent physio-mechanical characteristics that provide for
long-term mechanical stability
Low biodegradability
Zero toxicity
Lentikats Biocatalyst enables easy separation of enzymes and microorganisms from the surrounding media and therefore it can be reused multiple times.
Biotechnologies are changing
the world… 4

5. Lentikats Biocatalyst
Advantages of using PVA as a matrix for encapsulation:
inexpensive, non-toxic
polymerization under optimal conditions, cell conservation
enables creation of hydrogels with excellent mechanical properties
no side effects in processes
large capacity for cell building
easily supervised processes
stable processes
easy separation from bulk media

6. Lentikats Biocatalyst Production Lentikats Biocatalyst Application
Lentikats Lentikats Biocatalyst & Biotechnology (our product) (application process)
Lentikats Biocatalyst Production Lentikats Biocatalyst Application

7. How does Lentikats Biocatalyst function?
Due to its maximum surface and at the same time minimum volume of the Lentikats Biocatalyst, the diffusion limitations associated with the transport of the substrate into the lens and the product out of the lens are significantly reduced. 7

8. Production of Lentikats Biocatalyst8

9. Lentikats Biocatalyst
Production plant in Stráž pod Ralskem

10. Benefits of Lentikats Biotechnology

11. What are the advantages of Lentikats Biotechnology over traditional technologies?1)
Higher biomass concentration
Reduced volume of reactors
Reaction rate
Investment costs saving
Biomass concentration
100 €
100 €
100 €
100 €
Operational costs saving (electricity, heat, etc.)
Higher yields

12. What are the advantages of Lentikats Biotechnology over traditional technologies?
100 €
100 € 2)
Easy separation from bulk media
100 €
Operational costs saving
100 €
Repeatable use of Biocatalyst
Lower production of waste and by-products 3)
Robustness towards environmental shocks
profound change of pH level
higher concentration of toxic compounds
Applicability under extreme or stress conditions

13. Lentikats Biotechnology in distilleries

14. Technological process set-up
Legend:
Sugars media inlet
Fermented media
Inlet for Lentikats Biocatalyst filling
Outlet for Lentikats Biocatalyst emptying
Sieve separator (angle slop 15-20°, aperture mm)
Liquid fermentation broth with Lentikats Biocatalyst
Liquid fermentation broth without Lentikats Biocatalyst
Gear with speed change box
Stirrer
pH probe well
Temperature probe well
Antifoam agent inlet
Liquid level measurement (stainless steel pressure probe)

15. Which substrates can be used for Lentikats Biotechnology?
Lentikats Biocatalysts provide a solution for the production of bioethanol from the following substrates:
Molasses, beet juice and sugar cane
Starch-containing (cereal) materials (corn, wheat, etc.)
Ligno-cellulose containing materials (straw, pollard…)

16. Our products
Lentikats Biocatalyst for the fermentation of sucrose, glucose and lactose solutions (immobilized bacteria Zymomonas mobilis, yeast Saccharomyces cerevisiae, Kluyveromyces sp.)
Lentikats Biocatalyst for the 2nd step of hydrolyses of liquefied starch solution (immobilized enzyme glukoamylase)
Lentikats Biocatalyst for the production of inverted sugar from molasses (immobilized enzyme invertase)
Energy is all around us… …the key is to obtain it effectively… …and protect the environment at the same time…

17. β

18. Case study – molasses distillery

19. Molasses distillery (125,000 L A.A. per day, continuous mode)
45 tons of Lentikats Biocatalyst
(TFS* 48 %)
41 667  m3/year a.a.
404 tons/day
130 g/L TFS
* Total Fermentable Sugars

20. Fermentation conditions
Saccharomyces cerevisiae
continuous mode
180 g sucrose . hour-1 . kg-1
ethanol yield 95 %
8.4 vol. % ethanol

21. Economic comparison of traditional technology and Lentikats Biotechnology for molasses distillery
Biotechnologies are changing the
world…..

22. Reactor volume reduction – reduced investments
Traditional technology
Lentikats Biotechnology
Fermentation volume
3,450 m3
360 (2 x 180) m3
Reserve reactor -
180 m3
Pre-fermentation volume
213 m3
Volume for preparation of nutrients
16 m3
Equalisation tank before distillation
Sanitation tank
48 m3
Total
3,727 m3
768 m3
Production of ethanol
39,035,667 L/year
41,666,667 L/year
Process yield
290 L/tons
309 L/tons
Biotechnologies are changing the
world…..

23. Lentikats Biocatalyst consumption (125,000 L a.a. per day)
Biocatalyst type
Lifetime
Annual consumption of Lentikats Biocatalyst
Price (€/year)
immobilised yeast Saccharomyces cerevisiae
8*–12* months
61.5*–41** tons
790,000 –526,500
Biotechnologies are changing the
world…..
* Guaranteed lifetime ** Assumed lifetime
Biotechnologies that are environmentally friendly …

24. Profit of ethanol production at the capacity of 125,000 L a.a. per day
Traditional technology
Lentikats Biotechnology
Ethanol production (m3/year a.a.)
39,036
41,667
Ethanol yield from molasses (L/ton)
290
309
Annual revenues for ethanol (€)
24,600,000
26,500,000
Gross profit without depreciation (€)
7,250,000
8,185,000
Biotechnologies are changing the
world…..
Solution for higher efficiency and stability…

25. Case study – distillery processing sugar solutions (sugar cane or sugar beet juice)

26. Sugar distillery (125,000 L a.a. per day, continuous mode)
45 tons of Lentikats Biocatalyst
sugar solution
41 667  m3/year a.a.
140 g/L TFS *
* Total Fermentable Sugars

27. Fermentation conditions
Biotechnologies are changing the
world…..
Biotechnologies are changing the
world…..
Zymomonas mobilis
continuous mode
180g saccharose. hour-1 . kg-1
temperature: 30–35°C, pH 4.8–5
ethanol yield 95 %
9.02 vol. % ethanol

28. Economic comparison of traditional technology and Lentikats Biotechnology for distillery processing sugar solutions

29. Lentikats Biocatalyst consumption (125,000 L a.a. per day)
Biocatalyst type
Lifetime
Annual consumption of Lentikats Biocatalyst *
Price (€/year)
immobilised bacteria Zymomonas mobilis
1.5*–2** years
27.4*–20.5** tons
351,500
–263,500
Biotechnologies are changing the
world…..
Biotechnologies are changing the
world…..
* Guaranteed lifetime, ** assumed lifetime
Biotechnologies that are
environmentally friendly …

30. Profit of ethanol production at the capacity of 125,000 L a.a. per day
Traditional technology
Lentikats Biotechnology
Ethanol production (m3/year a.a.)
39,036
41,667
Annual revenues for ethanol (€)
24,600,000
26,500,000
Gross profit without depreciation (€)
7,240,000
8,630,000
Solution for higher efficiency and stability…

31. Case study – corn distillery

32. Corn distillery (15,000 L a.a. per day, continuous mode)
1.8 tons of LB
6 tons of LB
(60 % of starch)
5,000,000 m3/year a.a.
39 tons/day

33. Hydrolysis conditions
glucoamylase
continuous mode
600 g glucose . hod-1 . kg-1
temperature: 38–40°C, pH 4.5–5
conversion rate 95 %
outlet concentration 150 g/L

34. Fermentation conditions
Zymomonas mobilis
continuous mode
180g glucose. hour-1 . kg-1
temperature: 30–35°C, pH 4.8–5
ethanol yield 95 %
9.21 vol. % ethanol
Biotechnologies are changing the
world…..
Biotechnologies are changing the
world…..

35. Economic comparison of traditional technology and Lentikats Biotechnology for corn distillery

36. Reactor volume reduction – reduced investments
Traditional technology
Lentikats Biotechnology
Mixing tank
14 m3
Fermentation reactor
26 m3
Saccharification reactor
9 m3
36 m3
Fermentation volume
552 m3
72 (2 x 36) m3
Reserve reactor -
Pre-fermentation volume
86 m3
Sanitation tank
Equalisation tank before distillation
Total
696 m3
219 m3
Production of ethanol
4,473,667 L/year
5,000,000 L/year
Process yield
344 L/tons
385 L/tons

37. Lentikats Biocatalyst consumption (125,000 L a.a. per day)
Biocatalyst type
Lifetime
Annual consumption of Lentikats Biocatalyst *
Price (€/year)
immobilised enzyme glucoamylase
1,200*–1,500** hours
11.9*–9.5**
tons
150,000 –120,000
immobilised yeast Saccharomyces cerevisiae
1.5*–2**
years
3.6*–2.7**
46,000 –34,500
* guaranteed lifetime, ** assumed lifetime
Biotechnologies that are
environmentally friendly …

38. Profit of ethanol production at the capacity of 15,000 L a.a. per day
Traditional technology
Lentikats Biotechnology
Ethanol production (m3/year a.a.)
4,474
5,000
Ethanol yield (L/ton of corn)
344
385
Annual revenues for ethanol (€)
2,817,000
3,150,000
Gross profit without depreciation (€)
1,370,000
1,600,000
Solution for higher efficiency and stability…

39. Other Lentikats Biotechnology Applications
Lentikats Biocatalysts and applied Lentikats Biotechnology also deliver additional value and benefits in the following sectors :
waste water treatment – nitrogen removal
pharmaceutics – production of robust biocatalysts
food industry – whey processing, production of special sugars carbohydrates and syrups
Biotechnologies are changing the world…

40. What can we offer?

41. Lentikats Biotechnology services
Monitoring of specified technological procedures and conditions.
Periodical monitoring of Lentikats Biocatalyst’s activity.
Supply and replacement of Lentikats Biocatalyst in such periods and volumes to sustain the required Lentikats Biotechnology parameters.
Disposal of the used Biocatalyst.

42. What can we offer to you?
Have we raised your interest in testing Lentikats
Biotechnology in your own project?
We are prepared to supply a sufficient amount of our Biocatalyst and provide full assistance in order to convince you of the obvious advantages our technology has when applied in your distillery.
For all companies introducing new technologies, the first applications and end results are the most important ones. That is why we offer excellent conditions to potential clients in an effort to eliminate any of their risks.

43. Guarantee of your investments and the investment return
You can test our biocatalyst easily by yourself with your substrate in your own plant or plant of your choice to see the results.
We offer to you our assistance and full support at:
- pilot model installation
- installation into an existing reactor
Load of Biocatalyst for testing is free of charge.

44. References and experiences, our partners

45. Our know-how, references and experiences
Production of bioethanol from sugar beet molasses in Bioferm Kolín distillery with a capacity of 6 litres of absolute alcohol per hour.
Processing of technical-technological documentation for the supply to an agricultural refinery with a production capacity of cca m3 of bioethanol per year, based on our concept of Bio Plant 2015.
Processing of technical documentation for the delivery of a distillery with a production capacity of cca 450 hl of bioethanol per day utilising corn as a raw material.
Production of bioethanol from starch milk of type B in Krnov distillery with a capacity of 12 litres of absolute alcohol per hour.
Process optimisation and processing of technical documentation for a reconstruction of the existing distillery in India which uses cane molasses as a substrate.

46. Our partners in the field of industrial applications
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, India
VÚCHZ, a.s., Křižíkova 70, Brno, CZ
Gresham’s Eastern (Pvt) Ltd.
F-302 Sind Industial Trading Estate
Karachi
Zinchem, Námestie Slobody 5, Michalovce, SK sk
geniaLab®, BioTechnologie-Produkte und Dienstleistungen Gmbh Büchnerstraße 7, Braunschweig, Germany 46

47. Our partners in the field of research and development
ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, Praha 6, CZ
Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, India
CHEMCOMEX Praha, a.s., Pražská 810/16, Praha 10, CZ
Institute of Microbiology, Academy of Science of the Czech Republic Vídeňská 1083, Praha 4, Krč, CZ
STU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, Bratislava, SK
Association of Alcohol Producers in Slovakia, Záhradnícka 21, Bratislava, SK
Sekab, Box 286, s Örnsköldsvik, Sweden 47

48. Thank you for attention
Company Headquarters:
LentiKat´s a.s.
Evropska 423/178
Prague 6
Czech republic
Tel.:
Fax:
Production plant and laboratories
Pod Vinicí 83
Stráž pod Ralskem
Tel.:
Fax: 48 48