1. Interpenetrating Networks for Delivery Systems Client: Professor John W. Kao Advisor: Professor Kristyn Masters Claire Flanagan Ashley Huth Max Michalski Adam Rieves

2. Overview Problem Statement Background Information Design Constraints Design Approaches Preliminary Data Conclusions

3. Problem Statement Our goal is to create a novel delivery mechanism to reconstitute the components of an interpenetrating network (IPN).

4. Interpenetrating Network (IPN) Covalently Linked Therapeutic(s) and/or Cell Adhesion Ligands Bifunctional PEG Linkers Soluble Therapeutic(s) Biodegradable Gelatin Backbone PEG- diacrylate (2-3.4 kDa ) Solution (drugs + matrix component) in situ UV curing *Kao, W.J

5. Clinical Applications Issues Uneven administration Reconstitution Method Heat Time Shelf life IPN Conventional Dressings Irregular Wound Benefits Moist healing environment Conforms to irregular wounds Covers large surface areas Delivers drug cocktails Very biocompatible *Kao, W.J.

6. ingredients w/ drug(s) in one container Mix Ideal Clinical Administration 1 1 Cover 5 5 Shake 2 2 3 3 Spray with a controlled distribution over irregular wound surface 4 4 Cure in 30 sec to obtain a rubbery film 6 6 Sustained Release while the IPN biodegrades Day 7 Day 3 Day 1 7 7 Clean *Kao, W.J

7. Design Constraints Clinically applicable No special equipment required Simple reconstitution methods Administration via spray bottle Completely reconstitutes Meets viscosity requirements Cure time < 60 seconds Improves Shelf Life

8. Approach 1: Heating Element Exothermic Reaction Resistive Element Pros Feasibility Viscosity Passive Procedure Cons Cost Client Preference Safety

9. Approach 2: Research Alter pH Add Surfactants Try Buffer Varieties Pros Client Preference Reconstitution Time Cost Cons Feasibility Safety Active Procedure

10. Design Matrix

11. Preliminary Data **Water at 60 ºC

12. Preliminary Data **All solutions at room temperature **Final solution 10% gelatin

13. Conclusions We hope to further the clinical applications of IPNs Future Work Continue literature research Gather data Test complete IPNs Reconsider design approach

14. Questions