1. Cytotoxicity and Uptake of Nanoparticles in Cells Courtney Rister Faculty Advisors: Virginia Ferguson Conrad Stoldt Department of Mechanical Engineering University of Colorado, Boulder

2. October 15, 2004 Introduction to Research Presentation 2 Objective How does nanoparticle presence affect cellular function and behavior?  Little is known on how they interconnect with cytoskeleton Long term goals  Which nanoparticles are most efficient?  What is the best way to deliver the nanoparticles?

3. October 15, 2004 Introduction to Research Presentation 3 Background Uses of magnetic nanoparticles in bioengineering  Therapeutic  Hyperthermia  Drug Delivery  Diagnostic  Nuclear Magnetic Resonance (NMR)

4. October 15, 2004 Introduction to Research Presentation 4 Methodology Perform cell cultures with Mammalian SaOS-2 cells Obtain either Magnetite or Gadolinium nanoparticle cells Encapsulate nanoparticles in liposomes  Prevents formation of large aggregates and increases biocompatibility Introduce nanoparticles to SaOS-2 cells and record effect

5. October 15, 2004 Introduction to Research Presentation 5 Requirements TEM (Transmission Electron Microscope) BioServe equipment & supplies  Cells  Culture media and facilities

6. October 15, 2004 Introduction to Research Presentation 6 Timeline Oct.Nov.Dec.Jan.Feb.MarchAprilMay Literature Review Initial Project Design Cell Cultures Finalize Project Plans Finalize Experiment al Plans Perform Experiments