1. Langmuir technology

3. STM photo of Langmuir film of Stearic Acid C 17 H 35 COOH

4. Langmuir technology

5. Schemas of STM and AFM a – scanning tunneling microscopy b – atomic force microscopy

6. AFM images of Langmuir films of polymers Langmuir film of antibodies with polymer PEI-R12 on graphite surface Langmuir film of antibodies with polymer PEI-L70 on graphite surface. AFM image, size 2,6x2,6 μ.

7. AFM images of Langmuir films of polymers ? ab c d AFM images of Langmuir films of polymer PEI-R12, size1,5  1,5 μ. a – surface pressure by LB process  í =10 mN/m, thickness of film 2,5 nm; b –  í =20 mN/m, thickness of film 3,5 nm; c –  í =30 mN/m, thickness of film 3,5 nm; d –  í =40 mN/m, thickness of film 3,5 nm.

8. AFM images of Langmuir films of polymers AFM images of Langmuir films of polymer PEI-L70, size1,5  1,5 μ. a – surface pressure by LB process  í =10 mN/m, thickness of film 3,3 nm; b –  í =20 mN/m, thickness of film 4 nm; c –  í =30 mN/m, thickness of film 5,5 nm; d –  í =40 mN/m, thickness of film 6 nm. ab cd

9. STM images of DNA DNA molecules on graphite surface

10. AFM images of viruses Sample of rotaviruses specific adsorbed on Langmuir film from antibodies with polymer PEI-R12. AFM image, 3D projection with highlight. Viruses of Variolovaccine on mica surface

11. Cells of Rickettsia bacterium C. burnetii (culture ”Yellow throat mouse -Luga”) on Langmuir film from antibodies with polymer PEI-L70. 3D projection with highlight. Cells of bacterium Rickettsia prowazekii (culture ”Breil”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight. Cells of bacterium Rickettsia bacterium C. burnetii (culture ”M-44”) onmica. 3D projection with highlight. AFM images of bacteriums

12. Cells of bacterium Yersinia pestis (culture ”EB”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight. AFM images of bacteriums

13. ASM images of Bacillus subtilis AFM images of bacteriums

14. ASM images of Staphylococcus aureus 209-P

15. AFM images of bacteriums Bacteriums Staphylococcus aureus on glass. ASM image with highlight. Bacteriums Staphylococcus aureus, treated for 15 min by enzyme lysoamidaze. ASM image with highlight.

16. Investigations of 2D crystalline films of ferritin 1- initial electronmicroscopic photo 2 - optical diffraction 3 - after Fourier filtration 1 2 3

17. Investigations of 2D crystalline films of protein Self-organized protein of S-layer of Bac.spaericus cell wall with tetragonal packing of sub-units. TEM, contrast by 1% uranyle-acetate. 1 - fragment EM photo, 2 - optical diffraction pattern, 3 - filtered image 1 2 3 1 3 2