1. D.N.A
Describe how you would go about genetically engineering a bacterium to produce human epidermal growth factor (EGF), a protein used in treating burns. Use the following vocabulary words: DNA ligage, E.coli, plasmids, restriction enzymes

2. CLASSWORK Chapter 10 Review (1-7) Chapter 11 Review (1-8)

4. Protein Synthesis Jeopardy
Biotechnology
Transcription
RNA Processing
Translation
Mutations 10 20 30 40 50

5. This is used to cut DNA at a specific location for splicing
RESTRICTION ENZYME
Category

6. This occurs when DNA in which genes from different sources are combined
Recombinant DNA
Category

7. A small piece of bacterial DNA used for gene transfer
Plasmid

8. Electrophoresis is use to…1 2 3 4
Electrophoresis is use to…
Separate fragments of DNA

9. A genetic marker is…
A place where a restriction enzyme cuts DNA
A chart that traces the family history of a genetic trait
A nucleotide sequence near a particular gene
A radioactive probe used to find a gene
An enzyme used to cut DNA

10. This is the site of transcription in eukaryotes.
Inside the nucleus

11. This is the enzyme that carries out the process of transcribing RNA from the DNA molecule
RNA polymerase

12. Before leaving the nucleus as mRNA, extra nucleotides are added to the ends of the transcript, and noncoding regions called ______ are removed
INTRONS

13. A sequence of 3 bases on mRNA is also known as this.
CODON

14. This is the transcribed mRNA sequence for the following DNA strand:
‘3 T A C G A A 5’
A U G C U U

15. These types of cells do NOT undergo RNA processing
PROKARYOTE

16. These are the respective names of the protective segments added onto the 5’ leader and 3’ trailer end of the mRNA
Cap and Tail

17. These are the respective names of coding and non-coding segments of the pre-mRNA
Intron and Exons

18. This is the cutting and pasting process in which coding sequence is put together
RNA splicing

19. These are RNA molecules that act like enzymes and catalyze some RNA splicing

20. This is the site of translation in all cells
RIBOSOME

21. Translation of the “words” of the mRNA message into the ____________ sequence of a protein
Amino acids

22. During the process of translation, _______ matches a nucleic acid codon with the proper amino acid
Transfer RNA

23. This is the name for the 3 bases on the bottom of the tRNA molecule
ANTICODON

24. Explain the translocation step of TRANSLATION

25. Explain what base substitution is and give an example of a disease that is caused by this type of mutation
Replacement of one nucleotide with another – sickle cell anemia

26. Adding or the deletion of nucleotides may alter the ____________ (triplet grouping) of the message
Reading frame

27. What type of frameshift mutation is seen below?

28. Imagine an error occurring during DNA replication in a cell, so that where there is supposed to be a T in one of the genes there is instead a G. What effect will this probably have on the cell?

29. Which type of mutation – a base substitution or a base deletion is likely to have the greatest effect on the organism? Why?