1. The Classification Of Genus Bacillus
Liu Guohong

2. Content Characteristic of Bacillus
Related Bacillus and Its summarization
Fatty acid identification
The phenotypic characteristic of ten Bacillus species

3. Characteristic of Bacillus
Bacillus rod-shaped, Gram-positive or variable, spore-forming, aerobic or facultatively anaerobic.
The genus includes thermophilic and psychrophilic, acidophilic and alkalophilic, freshwater and halophilic bacteria that utilize a wide range of carbon sources for heterotrophic growth or grow autotrophically.

4. Colony roough, untransparent, cream-white or brown, drape, producing pigment.pH
G+C mol%.
Type species is Bacillus.subtilis.

5. Related Bacillus and Its summarization

7. Thermobacillus
Gram-negative, rod-shaped, aerobic,spore-forming, non-motile,
Main fatty acid is iso-C16:0. The G+C is 57.5 mol%.
Type species is Thermobacillus xylanilytus.
Geobacillus
Vegetative cell rod-shaped, single or in short chain, motile by means of peritrichous flagella or non-motile
aerobic or facultatively anaerobic.
37-75 ℃, pH
Main fatty acid is iso-C15:0, iso-C16:0 and iso-C17:0. The G+C is mol%.
Type species is Geobacillus stearothermophilus.

8. Ureibacillus
Colony cirluar,flat, transparent,motile,Gram-negative, rod-shaped or in chain, aerobic, thermopfilic.
Main fatty acid is iso-C16:0. The G+C is mol%.
Type species is Ureibacillus thermosphaericus.

9. Thiobacillus
Growth condition range is large, pH0-8, 20-50℃.
Colony is small, Gram-negative, motile.
G+C 50-68%.

10. The second edition of《Bergey's Manual of Systematic Bacteriology》, eight species of Thiobacillus were reassigned to three new genera，namely Acidithiobacillus (including Thiobacillus thiooxidans, Thiobacillus ferrooxidans, Thiobacillus caldus and Thiobacillus albertensis), Halothiobacillus (including Thiobacillus neapolitanus, Thiobacillus halophilus and Thiobacillus hydrothermalis) and Thermithiobacillus
(including Thiobacillus tepidarius).

11. Halothiobacillus
Gram-negative,rod-shaped, Obligately acidophilic, using reduced sulfur compounds to support autotrophic growth.
optimum tempaerate is ℃, pH
The G+C is 56-68%.
Type species is Halothiobacillus neapolitanus (formerly Thiobacillus neapolitanus)
Thermithiobacillus
Obligately aerobic, moderately thermophilic and chemolithoautotrophic, Gram-negative, non-sporeforming
Grow on reduced inorganic sulfur compounds;, unable to oxidize ferrous
iron.
G+C 66-67%.
The type species is Thermithiobacillus tepidarius (formerly Thiobacillus tepidarius).
Acidithiobacillus
Obligately chemolithoautotrophic Gram-negative, rods, obtaining energy from reduced inorganic sulfur compounds.
G+C mol%，
The type species is Acidithiobacillus thiooxidans (formerly Thiobacillus thiooxidans).

12. Gracilibacillus
Obligately aerobic.
Main fatty acid is anteiso15:0, The G+C is mol%.
The type species is Gracilibacillus halotolerans.
Salibacillus
The main fatty acid is iso15:0 and anteiso 15:0.
The type species is Salibacillus salexigens.
Brevibacillus
Rod-shaped,Gram-positive or variable，obligately aerobic.
Main fatty acid is iso-C15:0 and anteiso-C15:0, The G+C is mol%.
The type species is Brevibacillus brevis.

13. Aneurinibacillus
Main fatty acid is iso-C15:0, iso-C16:0and C16:0 , The G+C is mol%.
The type species is Aneurinibacillus aneurinolyticus.
Lentibacillus
Main fatty acid is anteiso-C15:0 and iso-C16:0. The G+C is 44 mol%.
The type species is Lentibacillus salicampi.
Jeotgalibacillus
Main fatty acid is iso-C15:0. G+C 44 mol%.
The type species is Jeotgalibacillus alimentarius.
Marinibacillus
Main fatty acid is anteiso-C15:0.
The type species is Marinibacillus marinus.

14. Methylobacillus
Main fatty acid is C16:0 and C16:1.
Paralactobacillus
G+C mol%
The type species is Paralactobacillus selangorensis.
Panenibacillus
Main fatty acid is anteiso-C15:0.
Anoxybacillus
G+C 42 mol%.
The type species is Anoxybacillus pushchinensis.
Virgibacillus
Main fatty acid is iso-C15:0 and anteiso-C15:0.

15. Fatty acid identification

17. The phenotypic characteristic of ten Bacillus species

18. 菌 名
megaterium 4617
Bacillus
Bacillus megaterium 4632
subtilis 4620
licheniformis 4648
licheniformis 4646
pumilus4657
cereus4645
Bacillus cereus
4634
atrophaeus
4643
sphaericus
4639
耐盐2% + 5% 7% -
10%
温度5 +- 10 50 55
pH5.7
V-P
接触酶 淀粉 水解

19. Thanking for your attention