1. Stephanie Constant / MITM Assistant Professor Dept. of Microbiology, Immunology and Tropical Medicine mtmslc@gwumc.edu 994-1138 Hookworm E/S products and host immune evasion and Cyclophilin-CD147 interactions in inflammation

2. Stephanie Constant / MITM Na Ac 80 45 55 30 21 14 66 97 116 200 Hsieh et al. 2004 J. Immunol. 173:2699 Loukas et al. 2005 FEMS 43:115 Funding: R01 AI-059280-01 PROJECT 1 Hookworm E/S products and host immune evasion

3. PROJECT 1 Hookworm E/S products and host immune evasion IFN- (pg/ml) 0 500 1,000 1,500 +IL2 +IL12 +ES+IL2 +IL12 +ES ES products augment IFN- production by >30 fold in human NK cells Stephanie Constant / MITM Egg- negative (USA) Egg- positive (Brazil) 0 25 50 75 Patient Category % bioES + NK cells Egg- negative (Brazil) Patients with active hookworm infection do not bind ES products ES binding ES products bind only to NK cells

4. Stephanie Constant / MITM Arora et al. 2005 J. Immunol. 175:517 Yurchenko et al. 2005 Immunology 117:301 Funding: R01 AI-067254-01 PROJECT 2 Cyclophilin-CD147 interactions in inflammation 050100500FMLP 0 1 2 3 4 5 6 7 CyPA (ng/ml) Chemotactic index MediumCyPA +  CD147 CyPA +isotype 0 1 2 3 4 5 *** Chemotactic index Extracellular cyclophilins induce leukocyte chemotaxis Cyclophilin-mediated chemotaxis is dependent on CD147 signaling

5. Stephanie Constant / MITM PROJECT 2 Cyclophilin-CD147 interactions in inflammation 0 0.5 1.0 1.5 2.0 2.5 3.1256.2512.52550 Methacholine (mg/ml) Ratio Penh baseline anti-CD147 isotype control ** Mouse model of allergic asthma Gwinn et al. 2006 J. Immunol. 177:4870