1. ABC Monomers Matthew Mancuso BEE 7600, Professor Dan Luo Department of Biomedical Engineering, Cornell University Presented Thursday March 17 th, 2011 DNA based Dendrimers, Barcodes, and

2. DNA as a Generic Material 1.) DNA Dendrimers 2.) DNA ABC Monomers

3. Fractals and Dendrimers The Bottom Line: Dendrimers closely approximate the perfect structure and self-similarity found in many fractals.

4. DNA Dendrimers The Bottom Line: DNA can be used as the monomers which compose a Dendrimer.

5. DNA Dendrimer Characterization The Bottom Line: High generation DNA dendrimers ( and Y-DNA) can be formed in high yield and purity.

6. DNA Dendrimer Application: DNA Nanobarcodes Y. Li, Y. T. H. Cu, and D. Luo, "Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes," Nat Biotech 23, 885-889 (2005). The Bottom Line: DNA Dendrimers can be made functional through the addition of Flurophores or other small molecules.

7. Nanobarcode Specificity The Bottom Line: Different ratios of fluorophores can be used as different “barcodes” for identification.

8. Nanobarcode Specificity Y. Li, Y. T. H. Cu, and D. Luo, "Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes," Nat Biotech 23, 885-889 (2005). The Bottom Line: Nanobarcodes can be created in different ratios and create real-color patterns for targeted detection.

9. Nanobarcodes and Microbeads The Bottom Line: Microbeads can be functionalized with one half of the detection sequence, further localizing detection reactions.

10. DNA ABC Monomers J. B. Lee, Y. H. Roh, S. H. Um, H. Funabashi, W. Cheng, J. J. Cha, P. Kiatwuthinon, D. A. Muller, and D. Luo, "Multifunctional nanoarchitectures from DNA-based ABC monomers," Nat Nano 4, 430-436 (2009). The Bottom Line: DNA dendrimers are created by growing off all three ends of a Y, then caping the product. ABC Monomers are created so they can polymerize in two directions.

11. DNA ABC Monomer Polymerization J. B. Lee, Y. H. Roh, S. H. Um, H. Funabashi, W. Cheng, J. J. Cha, P. Kiatwuthinon, D. A. Muller, and D. Luo, "Multifunctional nanoarchitectures from DNA-based ABC monomers," Nat Nano 4, 430-436 (2009). The Bottom Line: Polymerization can be used for detection too, possibly increasing the lower limit of detection.

12. Multiplexed Detection J. B. Lee, Y. H. Roh, S. H. Um, H. Funabashi, W. Cheng, J. J. Cha, P. Kiatwuthinon, D. A. Muller, and D. Luo, "Multifunctional nanoarchitectures from DNA-based ABC monomers," Nat Nano 4, 430-436 (2009). The Bottom Line: Multiplexed Detection is made possible by mixing monomers targeted at different DNAs.

13. DNA ABC Monomers J. B. Lee, Y. H. Roh, S. H. Um, H. Funabashi, W. Cheng, J. J. Cha, P. Kiatwuthinon, D. A. Muller, and D. Luo, "Multifunctional nanoarchitectures from DNA-based ABC monomers," Nat Nano 4, 430-436 (2009). The Bottom Line: ABC monomer spheres can be up taken in cells and display low cytotoxicity.

14. Thank You! The Bottom Line: DNA can be used as a generic material, even without the addition of nanoparticles, etc.