1. LAB 304 Lecture \ 9

2. Learning objectives To recognize urinalysis procedures: Physical Chemical Microscopic List some of urine crystals List some of urine casts.

3. Results interpreting

4. 1 2 3

5. 1- Physical examination Color Appearance Volume Specific gravity (SG)

6. NORMAL COLOR Including color and clarity Normal urine color ranges from pale yellow to deep amber

7. NORMAL COLOR

8. ABNORMAL COLOR Abnormal color : some drugs and urinary tract infection cause color changes reddish urine 1. reddish urine: hematuria, hemoglobinuria yellow-brown or green-brown urine 2. yellow-brown or green-brown urine: bilirubin cause : obstructive jaundice greenish urine 3- greenish urine : infection blue,orange,purple Other abnormal colors : blue,orange,purple

11. Appearance Clarity: normally, clear Abnormal appearance: POSSIBLE CAUSEAPPEARANCE BACTERIAL URINARY INFECTION CLOUDY 1- URINARY SCHISTOSOMIASIS 2- BACTERIAL INFECTION DUE TO RBCRED & CLOUDY 1- ACUTE VIRAL HEPATITIS 2- OBSTRUCTIVE JAUNDICE DUE TO BILIRUBIN YELLOW-BROWN OR GREEN-BROWN 1- HAEMOLYSIS 2- HEPATOCELLULAR JAUNDICE DUE TO UROBILINYELLOW-ORANGE

12. Urine volume The average adult : 1000ml to 2000ml/24h Increased POLYURIA---more than 2500ml of urine in 24 hours 1. physiological states: water intake, some drugs ( diuretics ), intravenous solutions 2. pathologic states: diabetes mellitus, diabetes insipidus

13. Urine volume Decreased OLIGURIA : less than 400ml of urine in 24 hours ANURIA : less than 100ml of urine in 24 hours 1. pre-renal: hemorrhage, dehydration, congestive heart failure 2. post-renal: obstruction of the urinary tract (may be stones, carcinoma)

14. Specific gravity (SG) Reflect the density of the urine Range of 1.001 to 1.040 Increased: Dehydration, Fever, Vomiting, Diarrhea, Diabetes Mellitus (urine volume ↓ and SG ↑ ) Decreased: diabetes insipidus (urine volume ↑ and SG ↓ )

15. 2-Chemical examination Urine PH Protein Glucose Ketones Occult blood Bilirubin Urobilinogen Nitrites

18. Urine PH Normal PH The average is about 6 Range from 5~7 (depends on diet) Higher PH---alkaline urine ( 7.8 – 8.0 ) 1.drugs: sodium bicarbonate 2.vegetarian 3.alkalosis (metabolic or respiratory) 4. urinary tract infection Lower PH---acid urine ( 4.5 – 5.5 ) 1. drugs: ammonium chloride 2. diabetes 3. acidosis (metabolic or respiratory)

19. Protein in urine Reference value Qualitative method: negative Quantitative method: ＜ 150mg of protein in 24 hours Urine proteins come from plasma proteins e.g. albumin and Tamm-Horsfall (T-H) glycoprotein

20. Protein in urine The two most common risk factors for proteinuria are: 1. Diabetes 2. hypertension Proteinuria---- ＞ 150 mg /24 hours or qualitative test is positive Proteinuria quantification (amount of protein ) heavy proteinuria---- ＞ 4 g/24 hours moderate proteinuria----1 - 4 g/24 hours minimal proteinuria---- ＜ 1 g/24 hours

21. Protein in urine TYPE PATHOPHYSIOLOGIC FEATURES CAUSE Glomerular glomerular capillary permeability to protein Primary or secondary glomerulopathy e.g. IgA nephropathy, lupus nephritis Tubular tubular reabsorption of proteins in glomerular filtrate Tubular or interstitial disease due to: Uric acid nephropathy Heavy metals, NSAIDs Overflow production of low-molecular-weight proteins Monoclonal gammopathy, leukemia Classification of Proteinuria

22. Glucose in urine Reference value Qualitative method: negative Glycosuria--- qualitative test is positive 1. with hyperglycemia ( most common ): diabetes mellitus, Cushing’s syndrome 2. without hyperglycemia (Renal glycosuria ): renal tubular dysfunction, such as pyelonephritis

23. Ketones in urine Including three ketone bodies: 1. acetone 2% 2. acetoacetic acid 20% 3. β-hydroxybutyric acid 78% The products of fat catabolism ( breakdown ) Reference value: qualitative method: negative Ketonuria--- qualitative test is positive

24. Ketones in urine Ketonuria 1. diabetic ketonuria : 1. diabetic ketonuria : I. Poorly controlled diabetes II. Diabetic ketoacidosis (DKA) 2. nondiabetic ketonuria: 2. nondiabetic ketonuria: I. Acute or severe illness II. Burns III. Fever IV. Hyperthyroidism V. Pregnancy & lactation VI. Abnormal food or nutrition intake due to: Anorexia, fasting, high protein or low carbohydrate diets, starvation, vomiting over a long period of time

25. 3-Microscopic examination Sample preparation 1- Obtain fresh urine sample 2- shake the container to mix the sample 3- pipette suitable amount to test tube 4- Centrifuge it at 1500 to 3000 rpm for 5 minutes 5- Decant supernatant part 6- from the sediment Place 1 drop of urine on slide and apply cover slip 7- examine it under microscope ( 10x, 40x )

26. Microscopic examination Examination A- Cells B- Bacteria C- Crystals D- Casts

27. Microscopic examination A- Cells : 1- White Blood Cells (pus cell) Normal <2/ HPF in men and <5/ HPF in women Few : up to 10/ HPF Moderate : 11-40 / HPF Many : > 40 / HPF HPF = high power field

28. Microscopic examination 2- Red Blood Cells : smaller and more refractile than white cells Normal <3/ HPF Dysmorphic RBCs suggest glomerular disease

29. Microscopic examination 3- Epithelial cells : 3 types 1. Transitional epithelial cells are normally present

30. Microscopic examination 2. Squamous epithelial cells suggest contamination

31. Microscopic examination 3. Renal tubule epithelial cells suggest renal disease

32. Microscopic examination B- Bacteria : B- Bacteria : Diagnostic for Urinary Tract Infection Men: Any bacteria Women: 5 or more bacteria per HPF

33. Microscopic examination C- Crystals 1- Calcium oxalate crystals (square envelope shape)

34. Microscopic examination 2- Triple phosphate crystals (coffin lid shape) Associated with increased Urine pH (alkaline) Associated with Proteus Urinary Tract Infection

35. Microscopic examination 3- Uric Acid crystals (diamond shape)

36. Microscopic examination D- Casts 1- Epithelial cell casts of renal tubule

37. Microscopic examination 2- Red Blood Cell casts

38. Microscopic examination 3- White Blood Cell casts

39. Microscopic examination 4- Hyaline or mucoprotein casts

40. Microscopic examination 5- Granular casts

41. Microscopic examination 6- Waxy casts

42. Microscopic examination 7- Fatty casts Oval fat bodies ( OFB )