1. Protein and cell nano-structures

3. Electron Carriers Function in Multienzyme Complexes The electron carriers of the respiratory chain are organized into membrane- embedded supramolecular complexes that can be physically separated.

4. Components of the respiratory chain in mitochondria,

5. Path of electrons from: NADH, succinate, fattyacyl–CoA, glycerol 3-phosphate To ubiquinone

7. Complex I: NADH to Ubiquinone NADH: ubiquinone oxidoreductase or NADH dehydrogenase A large enzyme composed of  42 different polypeptide chains, (including an FMN-containing flavoprotein)  at least six ironsulfur centers. L-shaped with one arm of the L in the membrane and the other extending into the matrix

8. The complex is a dimer of identical monomers, each with 11 different subunits. Structure of a monomer (PDB ID 1BGY).. The functional core is three subunits:  Cytochrome b (green) with its two hemes (bH and bL, light red);  The Rieske iron-sulfur protein (purple) with its 2Fe-2S centers (yellow);  Cytochrome c1 (blue) with its heme (red)

9. The dimeric functional unit The iron-sulfur protein (ISP) component of cytochrome bc1 complex was first discovered and isolated by John S. Rieske and co-workers in 1964 Antimycin A, which blocks electron flow from heme bH to Q, binds at QN, Myxothiazol, which prevents electron flow from QH2 to the Rieske iron-sulfur protein, binds at QP,

10. The Q cycle. The path of electrons through Complex III is shown by blue arrows

11. Complex IV: Cytochrome c to O2 cytochrome oxidase, carries electrons from cytochrome c to molecular oxygen, reducing it to H2O. Complex IV is a large enzyme (13 subunits; Mr 204,000) Bacteria contain a form that is much simpler, with only three or four subunits, Comparison of the mitochondrial and bacterial complexes suggests that: three subunits are critical to the function

12. Subunit I (yellow) has two heme groups, a and a3 (red), a copper ion, CuB (green sphere). Hemea3 and CuB form a binuclear Fe-Cu center. Subunit II (blue) contains two Cu ions (green spheres) complexed with the-SH groups of two Cys residues in a binuclear center, CuA center Subunit III (green) seems to be essential for Complex IV function, but its role is not well understood.

13. The binuclear center of CuA.. Ligands around the Cu ions include:  two His (dark blue),  two Cys (yellow),  an Asp (red),  Met (orange) residues.

15. ATP Synthesis How is a concentration gradient of protons transformed into ATP? PMF drives the synthesis of ATP as protons flow passively back into the matrix through a proton pore associated with ATP synthase. Mitchell used “chemiosmotic” to describe enzymatic reactions that involve, simultaneously, a chemical reaction and a transport process. ADP + Pi ATP nH + P nH + N

16. Chemiosmotic model

17. ATP Synthase Has Two Functional Domains, Fo and F1 Mitochondrial ATP synthase is an F-type ATPase F1, the first factor recognized as essential for oxidative phosphorylation, was identified and purified by Efraim Racker and his colleagues in the early 1960s. Isolated F1 catalyzes ATP hydrolysis (the reversal of synthesis) and was therefore originally called F1ATPase. When purified F1 is added back to the depleted vesicles, it reassociates with Fo, plugging its proton pore

18. Mitochondrial F1 9 subunits 5 different types, with the composition

19. The crystallographic determination of the F1 structure has been done by John E. Walker Although the amino acid sequences of the three subunits are identical, their conformations differ, in part because of the association of the γ subunit with just one of the three. The single γ subunit associates primarily with one of the three ά β pairs, forcing each of the three β subunits into slightly different conformations, with different nucleotide-binding sites. There is difference in nucleotide binding among the three subunits of β subunits which is critical to the mechanism of the complex.

20. If 10 protons are pumped out per NADH and 4 must flow in to produce 1 ATP, the proton-based P/O ratio is 2.5 (10/4) for NADH as the electron donor And 1.5 (6/4) for succinate. In previous the overall reaction equation would take the following form: with the value of x—sometimes called the P/O ratio or the P/2e ratio—always an integer. Most experiments have yielded ratios of between 2 and 3 when NADH was the electron donor, and between 1 and 2 when succinate was the donor.

21. The Proton-Motive Force Energizes Active Transport adenine nucleotide translocasephosphate translocase &

23. The inside of a bacterial cell is typically at an electrical potential about 150 mV below the outside, and also has a slightly lower concentration of H + or Na + ions. Filaments rotate at speeds up to 1000 Hz in swimming cells If cells are attached to a surface by a single flagellar filament, or “tethered”, the motor turns the whole cell body at speeds around 10 Hz

24. STRUCTURE AND COMPOSITION OF THE FLAGELLAR MOTOR

25. Composite electron micrograph of the flagellum basal body and hook, produced by rotational averaging (Francis et al., 1994). The motor proteins and export apparatus do not survive the extraction procedure and so are not shown. Image courtesy of David DeRosier, reproduced with permission.

27. About 60,000 Ribosomes (Small Yellow dots)

29. Transcription Translation Protein Synthesis

31. Ribosome, mRNA, sRNA, tRNA Protein Synthesis

32. Ribosome Binding mRNA

35. Ribosome 3-D Structure Complexity