1. Summary of electron transport
There can be branches, at terminal electron acceptor, at terminal oxidase, at entry point of NADH (ie. hot stinking plants)

2. Unfortunately, oxygen is not just a terminal electron acceptor

3. NADH, a great source of energy
NADH H+ + ½ O2  NAD H+ + H2O
Highly exergonic; DGo = -220 kJ/mol
Actually in cell, much NADH than NAD, making the available free energy more negative
Much of this energy is used to pump protons out of the matrix

4. Pumping protons lowers the pH and generates an electrical potential

5. Generation of a proton-motive force
In an actively respiring mitochondria, the pH is ~0.75 units lower outside than in the matrix
Also generates an electrical potential of 0.15 V across the membrane, because of the net movement of positively charged protons outward across the membrane (separation of charge of a proton without a counterion)
The pH difference and electrical potential both contribute to a proton motive force

6. Really, what does that mean?
Energy from electron transport drives an active transport system, which pumps protons across a membrane. This action generates an electrochemical gradient through charge separation, and results in a lower pH outside rather than in. Protons have a tendency to flow back in to equalize the pH and charge. This flow is coupled to ATP synthesis.

7. Measuring the proton motive force
DmH = Dy – 2.3RTDpH/F
(different in Lehninger)
mH is the resulting proton motive force (sometimes p)
y is the electrochemical membrane potential
pH has a negative value, thus contribution is positive in this equation

8. Don’t get bogged down in the math, but …
(under standard conditions) DmH = V
Plug into DGo = -nFDEo and DGo = ~20 kJ/mole H+
The bottomline 

9. The proton motive force bottom line
Two components to energy derived from electron transport, pH and electrical potential. The electrical potential is the primary contributor to free energy.
Most of the energy from oxidation of NADH is conserved in the proton gradient

10. So what is the proton motive force used for?

11. Introducing ATP synthase

12. Electron transfer and ATP synthesis are coupled
ATP synthesis occurs only if electron transfer does, and vice-versa
When isolated mitochondria are suspended in buffer containing ADP, Pi and an oxidizable substrate (succinate) three things happen
Substrate is oxidized
Oxygen is consumed
ATP is synthesized

13. All components are essential
If ADP were omitted, no ATP synthesis would occur and electron transfer to oxygen does not proceed, as well.
Black – oxygen consumption
Red – ATP synthesis

14. There are compounds that can inhibit ATP synthesis
The antibiotic oligomycin binds to ATP synthase and inhibit it’s action.
By stopping ATP synthesis, this compound also stops electron transport.
Because oligomycin is specific for ATP synthase and not the various electron carriers, this inhibition supports the coupling of ATP synthesis to electron transport

15. There are compounds that can uncouple ATP synthesis from electron transport
DNP and FCCP block ATP synthesis, while permitting continued electron transport to oxygen – they are uncouplers
They pick up protons from the outside, diffuse in (they are hydrophobic so can pass through the membrane), and release proton back inside.
Electrons are still passed through the electron transport chain, but the proton gradient is destroyed.

16. Evidence for uncoupling

17. ATP synthase – A molecular machine

18. Something we’ll cover when we talk about enzymes in detail:
ATP synthase stabilizes ATP relative to ADP + Pi by binding ATP more tightly, this results in a free energy change that is near zero
This is an important point, but ignore for the most part now as we will cover this in detail later
What’s important now is that this reaction ATP synthesis from ADP and Pi occurs without a huge input of energy – you’ll see it is just mechanical energy.

19. ATP synthase has two functional domains
This enzyme has two distinct parts, one a peripheral membrane protein (F1) and one a integral membrane protein (Fo) ( the o stands for oligomycin sensitive)
These parts can be separated biochemically, and isolated F1 catalyses ATP hydrolysis (it has the site for ATP synthesis and hydrolysis)

20. The F1 component
This component is made up of nine proteins of five different types with a composition of: a3b3gde
Each of the three b subunits have a catalytic or “active” site where the reaction occurs
ADP + Pi  ATP + H2O

21. The a and b subunits make a cylinder with the g subunit as an internal shaft

22. Conformational changes
Although the b subunits have the exact same amino acid sequence and composition, they are in different conformations due to the g subunit.
These conformational differences affect how the enzyme binds ATP and ADP

23. The Fo component forms a proton pore in the membrane

24. Rotation of the g subunit by H+ translocation drives ATP synthesis
Passage of protons through the Fo component causes g to rotate in that internal chamber
Each rotation of 120o causes g to contact another b subunit, this contact forces b to drop ATP and stay empty
The three b subunits interact so that when one is empty, one has ADP and Pi, while another has ATP.

25. Proton transfer is converted to mechanical energy, then chemical energy

26. ATP synthase – at work

27. ATP exits the mitochondria through active transport
P N
Side Side

28. Regulation of ETC
Rate of mitochondrial respiration controlled by ADP availability ([ATP]/[ADP][Pi])
IF1 can bind and block ATP synthase at low pH
Hypoxia influences gene expression

30. Coordinated regulation – more on this later, but think about global effects

31. What happens when…
Cells increase NADH oxidation using alternative NADH oxidase?
Cells using lots of ATP