1. Historical Ethnobotany

2. Hildegard of Bingen 1098 - 1179

3. Joseph Smith 1805 - 1844

4. Fertile Crescent

5. King Assurbanipal – 668-626 BCE In his garden with Queen and Servants

6. Babylonian Medicine

7. Datura stramonium

8. Cannabis sativa

9. Mandrake – Atropa mandragora

10. ca. 1474

11. Water lily – Nymphaea alba

12. Vitis vinifera var. Pinot Noir

13. Opium poppy – Papaver somniferum

14. Ergot – Claviceps purpurea

15. Fly agaric – Amanita muscaria

16. Amanita muscaria ornaments?

17. Sumerian Headdress

18. Sun god Horus and Tuth-Shena

19. Urgent need to study medicinal plants 1.To rescue knowledge in imminent danger of being lost Inventory by WHO found 20,000 plant species in use for medicine in 90 countries Only 250 of those species are commonly used or have been checked for main active chemical compounds

20. Urgent need to study medicinal plants 2.The utility of plants in current therapy There has been a rush to develop synthetic medicines based on plant medicines, but often the synthetic medicines don’t work as well as the original plant medicines. For example – quinine and malaria

21. Efficacy of Quinine Quinine is traditional and effective preventative of malaria Synthetic preventatives such as chloroquine, maloprim, and fansidar have largely replaced the use of quinine Many strains of Plasmodium have developed resistances to the synthetics and the synthetics are more toxic. It is recommended that people do not take fansidar for more than 3 months due to potential liver damage.

22. Malaria Cycle

23. Anopheles freeborni mosquito – intermediate host and vector for Plasmodium sp.

24. Historical distribution of Malaria

25. Red areas show countries with malaria today

26. One of the sources of Quinine – Cinchona succirubra

27. Cinchona pubescens

28. Timeline of Quinine Use 1633, a Jesuit priest named Father Calancha described how to use quinine bark to cure fevers 1645 Father Bartolome Tafur took some bark to Rome and many of the clergy used it Cardinal John de Lugo wrote a pamphlet to be distributed with the bark - use of the bark became so widespread that in the papal conclave of 1655 no one died of malaria 1654 – English aware of use of quinine bark 1735, a French botanist named Joseph de Jussieu journeyed to South America and found and described the tree that is the source of the bark - he sent samples to Sweden where in 1739, Carl Linneaus named the tree genus Cinchona

29. Timeline of Quinine Use 20 to 40 species of Cinchona - the species are very hard to tell apart and the species will hybridize, so the exact number of species is unknown – mostly understorey trees 1820 the French chemists Joseph Pelletier and Joseph Caventou isolated the alkaloid quinine from the bark and identified it was the active ingredient in Peruvian bark 1861, an Australian named Charles Ledger obtained seeds from an Aymara Indian named Manuel Incra by 1930, the Dutch orchards in Java produced 22 million pounds of quinine, 97% of the world’s market

30. Chemical structure of quinine

31. Properties of Quinine Quinine itself is an odorless white powder with an extremely bitter taste It can be used to treat cardiac arrhythmias as well as malaria - it is also used as a flavoring agent Quinine prevents malaria by suppressing reproduction of the Plasmodium and also helps prevent some of the fevers and pain associated with malaria

32. Quinine fluoresces under UV light

33. Raymond Fosberg in the field in 1948

34. Cinchona bark drying in the sun in Ecuador, 1944

35. Turriabla, Costa Rica agricultural center

36. Urgent need to study medicinal plants 3. To find new molecular models in plants Many times we can take a plant chemical and modify it or make synthetic copies of it that are very valuable to us.

37. Lippia dulcis – sweetener from Pre-Columbian America

38. Hernandulcin

39. Lippia as a sweetener In Pre-Columbian America, several plants of the genus Lippia were used as sweeteners. (F. Verbenaceae – the verbenas). In the 20th century, L. dulcis was chemically analyzed and a new sweetener was found, hernandulcin, that is 800 to 1000 times sweeter than sucrose.

40. Urgent need to study medicinal plants 4. The wide use of plants in folk medicine One positive aspect of the use of medicinal plants is their low cost compared to the high price of new synthetic drugs that are totally inaccessible to the vast majority of the world’s people. Another benefit is that most medicinal plants don’t have the kinds of harmful side effects seen with synthetic drugs.

41. Diospyros lycioides – source of chewing sticks in Namibia

42. Ceanothus americanus – Native American chewing stick

43. Modern Chewing Sticks Most chewing stick plants have a wide range of antibacterial activity against a number of odontopathic bacterial species, and many also contained healing and/or analgesic compounds

44. Bloodroot – Sanguinaria canadensis

45. Rhizome of Bloodroot

46. Bloodroot extracts to treat dental plaque Bloodroot extracts have been identified as potentially valuable in controlling plaque Blood root has many alkaloids, known as sanguinaria alkaloids, and sanguinarine in particular, is thought to be a potential problem limiting the usefulness of blood root as a dental medicine There is an indication that sanguinarine may provoke glaucoma in predisposed humans and cats.