1. 【 Supplement methods 】 Real-Time Reverse Transcription-PCR Total RNA was isolated from HRGEc using the RNeasy® Mini Kit. cDNA was synthesized using Super-Script® III reverse transcriptase according to manufacturer instructions. Real-time PCR analyses were performed using TaqMan® Gene Expression Assays, an ABI Prism® 7900HT Sequence Detection System, RQ Manager 1.2 and SDS, version 2.3 (SABiosciences™). Each sample was tested in duplicate. Using real-time PCR data, gene expression was quantified and calculated relative to glyceraldehyde- 3-phosphate dehydrogenase.

2. 【 Supplement Figure 1 】 Measuring procedure of positive area by image analysis Positive area ratio (%) = (B)/(C) x 100 (A): Immunostaining of COL type VI. (B): Extracted positive area (white image) of COL type VI in glomerulus. (C): Extracted inside area of Bowman’s capsule. 2 ABC

3. 3 Before MMc stimulation 4 hours12 hours 24 hours COLVI γH2AX 【 Supplement Figure 2 】 Immunocytochemistry of HRGEc by COL type VI, g-H2AX and Merge over time ： Expression of COL type VI and g-H2AX in HRGEc before and 4,12 and 24 hours after MMc treatment were examined by immunocytochemistry. Before the MMc treatment, about 90% of the cells were g-H2AX (-)/ COL type VI (+). The number of g-H2AX (+) cells was slightly increased after 2 hours’ MMc treatment, and the majority of cells were g-H2AX-positive after 8 hours’ MMc treatment.

4. 【 Supplement Figure 3 】 Real-Time Reverse Transcription-PCR: COL type VI α1-3 mRNA were existed in HRGEc. (A) COL type VI α1-3 were synthesized in normal cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was detected as positive control for Real-time Reverse Transcription-PCR. Red line was indicated as GAPDH, and blue lines were COL type VI a1-3. (B) Average Ct was each 27.295, 25.754, 27.178, 26.104 in GAPDH and COL type VI a1-3. COL type VI α1-3 mRNA were synthesized in HRGEc. AB