1. Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique Check results, repeat once from your plate if necessary Red = S. marcescens / Small yellow = M. luteus / Cream = E. coli Exercise 11: Smear Preparation Each person make smear slides: ***Less is More!!!! Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9) Interesting colonies (2-5) from ubiquity home plates (Exercise 7) Exercise 12: Simple Staining Each person stain heat-fixed smear slides from Exercise 11 ***All stain and rinse water into tray: nothing down sink!!! Hint for microscopy: Best smears will be barely visible to the naked eye when complete. In order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus. Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample) !!!CLEAN YOUR SCOPE WELL!!!

2. Bacterial Shapes Coccus Cocci Diplococci Tetrad Streptococci Staphylococci Bacillus Bacilli Coccobacilli Streptobacilli Fusiform Spiral Vibrio Spirillum Spirochaetes Lab book pg 101 Sup. packet pg 56

3. No big blobs! Target circle optional: spread out wide to make smear as thin as possible Must be COMPLETELY dry! Water first always with solid media Figure 11.1

4. paper towel Figure 12.2

5. Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique Check results, repeat once from your plate if necessary Red = S. marcescens / Small yellow = M. luteus / Cream = E. coli Exercise 11: Smear Preparation Each person make smear slides: ***Less is More!!!! Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9) Interesting colonies (2-5) from ubiquity home plates (Exercise 7) Exercise 12: Simple Staining Each person stain heat-fixed smear slides from Exercise 11 ***All stain and rinse water into tray: nothing down sink!!! Hint for microscopy: Best smears will be barely visible to the naked eye when complete. In order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus. Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample) !!!CLEAN YOUR SCOPE WELL!!!