1. OnSite
HEV Rapid Test

2. Hepatitis A and Hepatitis E
But they are different diseases!
Similarities
Acute viral hepatitis
Transmitted via
fecal-oral route
Jaundice in 50% cases
Clinical symptoms
Supportive therapeutics

3. Different Viral Family
Hepatitis A Virus
Hepatitis E Virus
ssRNA picornavirus
Single serotype worldwide
Vaccine is available
ssRNA hepevirus
1 serotype, 4 genotypes
Vaccine is being developed

4. Different Transmission Routes
Hepatitis A
Hepatitis E
Contaminated food, water
infected food handlers
raw shellfish
Contaminated food and water
Rarely from person to person
Close personal contact
household contact
homosexual in men
child day care centers
Possible zoonotic transmission
Rarely by blood exposure
intravenous drug use
blood transfusion

5. Different Incubation Times
Hepatitis A
Hepatitis E
15-60 days, average 50 days
15-45 days, average 30 days

6. Different Population Hepatitis A Hepatitis E
Symptomatic infection is common in young adults between yrs
Affects all age groups,
Particularly common in
children & young adults
Genotype 1: yrs
Genotype 3-4: Japanese >60 yrs
Genotype 1-2: human
Genotype 3-4: human & animal
The most common acute
viral hepatitis.
Asymptomatic or atypical infection seen mostly in children
Found primarily in Asian, Africa
and South America

7. Different Disease Course
Hepatitis A
Hepatitis E
Mild illness
Mild illness in most cases
Complications:
Fulminant hepatitis
Hepatic cirrhosis in these with immuno-
compromised conditions
Complications:
Fulminant hepatitis
Cholestatic hepatitis
Relapsing hepatitis
1-2% fatality rate, but could go
up to 75% with chronic liver disease
20% in the 3rd trimester pregnant
women
up to 33% of premature infants
0.4% fatality rate
Self limiting,
no reports of chronic status
Self limiting, chronic status in these with
immuno-compromised conditions
HAV and HEV co-infection in young children may lead to LIVER FAILURE

8. Different IgM Generation Time
Hepatitis A
Hepatitis E
Appears in the 1st week
of infection
Peaks at 4 weeks
Detectable up to 2-3 months
Appears in the 2nd week
of infection
Peaks at 7 weeks
Detectable up to 4 months
IgM detection is the most common specific diagnostics

9. Current Clinical Status of HAV and HEV
HEV is often under diagnosed or misdiagnosed:
HAV is the most common acute viral hepatitis
Lack of awareness of the differences between HEV and HAV
Lack of awareness = Diagnostic kits are unavailable, in particular, HEV test kits are not well-known on the market
HEV is more severe than HAV in high risk groups &
requires prompt action to prevent disease progression
HAV and HEV co-infection in young children may lead
to liver failure!
Differential diagnosis of HEV and HAV will save lives

10. CTK Biotech Offers To aid in differential diagnosis of HEV and HAV
Easy to use screening kits
OnSite HAV IgM Rapid Test kit
OnSite HEV IgM Rapid Test kit
Complimenting confirmation kits
HAV IgM ELISA kit
RecimbilisA HEV IgM ELISA kit
To aid in differential diagnosis of HEV and HAV

11. OnSite HAV IgM Rapid Test Procedure
1 drop of
specimen
As easy as “1-2-3”
Minimal training required
No equipment needed
Fast and reliable result in
15 minutes
Can be used in any setting
1 drop of
sample diluent
Result
in 15 minutes!

12. OnSite HAV IgM Rapid Test CE
Test Result Interpretation
Negative
No HAV Infection
Positive
Current infection or
late stage of infection
Invalid
Repeat test

13. OnSite HAV IgM Rapid Test CE
CTK HAV IgM Rapid Test
Reference
Positive
Negative
Total 21 1 22
178
179
200
Performance
Only 15 minutes
to get results….
Relative sensitivity: 95.5%
Relative specificity: 100%

14. CTK HAV IgM ELISA Kit CE
Catalog: E0100
ADAPTABLE TO AUTOMATED SYSTEMS

15. CTK HAV IgM ELISA kit CE Performance CTK HAV IgM ELISA Reference
Positive
Negative
Total 39
361
400
Only 70 minutes
to get results….
Relative sensitivity: 100%
Relative specificity: 100%

16. OnSite HEV IgM Rapid Test CE
Catalog: R0095C

17. OnSite HEV IgM Rapid Test Procedure
1 drop of
specimen
As easy as “1-2-3”
Minimal training required
No equipment needed
Fast and reliable results in
15 minutes
Can be used in any setting
1 drop of
sample diluent
Result
in 15 minutes!

18. OnSite HEV IgM Rapid Test Result
Test Result Interpretation
Negative
No HEV Infection
Positive
Current infection or
late stage of infection
Invalid
Repeat test

19. OnSite HEV IgM Rapid Test CE
Performance
CTK HEV IgM Rapid Test
Reference
Positive
Negative
Total
314 6
320
734
740
1060
Only 15 minutes
to get results….
Relative sensitivity: 98.1%
Relative specificity: 99.2%

20. RecombiLISA HEV IgM ELISA Kit CE
Catalog: E0105
ADAPTABLE TO AUTOMATED SYSTEMS

21. RecombiLISA HEV IgM ELISA kit CE
Performance
CTK HEV IgM ELISA
Reference
Positive
Negative
Total 25
375
400
Only 75 minutes
to get results….
Relative sensitivity: 100%
Relative specificity: 100%

22. Final Messages to Doctors
HAV and HEV seem similar BUT are clinically different
HEV could be life threatening and requires
prompt action to prevent disease progression
HEV is often under diagnosed or misdiagnosed
Differential diagnosis of HEV and HAV is clinically significant

23. Final Messages to Doctors
For easy, on site screening & differentiation of HAV & HEV
Order CTK Biotech’s
OnSite HAV IgM Rapid Test
Cat: R0090C
OnSite HEV IgM Rapid Test
Cat: R0095C

24. Final Messages to Doctors
For further confirmation & differentiation of HAV & HEV
Order CTK Biotech’s
HAV IgM ELISA kit
Cat: E0100C
HEV IgM ELISA kit
Cat: E0105C

25. Early Diagnosis for a better life Together, we grow Athenese Dx & CTK

26. Together, we grow Athenese Dx & CTK
Athenese DX contact information:
Module # 407 & 408, 4th Floor,
Ticel Bio Park II, No. 5, CSIR Road,
Taramani, Chennai ,
TamilNadu, India.
Together, we grow Athenese Dx & CTK