1. AP Bio Ch 6 GoalsCellular Respiration Diagram the flow of energy through the biosphere. Describe the overall summary equation for cellular respiration. Explain why cellular respiration is exergonic. List the 3 metabolic stages of cellular respiration. Distinguish between substrate level phosphorylation & oxidative phosphorylation. Define oxidation & reduction, oxidizing agent & reducing agent. Explain why glucose doesn’t spontaneously combust in our cells. Explain the importance of phosphorylation. List the 2 coenzymes involved in cellular respiration & explain their roles. Explain the role dehydrogenases in the oxidation of glucose. Write a summary equation for glycolysis & describe where it occurs in the cell. Explain why glycolysis alone is not efficient. Describe the structure of the mitochondrion. Describe where pyruvate is oxidized to acetyl CoA, what molecules are produced, and how it links glycolysis to the Krebs cycle. Describe the locations, molecules in and molecules out for the Krebs cycle.

2. Explain at what point during cellular respiration glucose is completely oxidized. Explain the composition, location and function of the electron transport chain. Describe the process of chemiosmosis. Trace the path of electrons during aerobic respiration (cellular respiration). State what energy source most directly drives ATP synthesis during respiratory oxidative phosphorylation. Summarize the net ATP yield from the oxidation of a glucose molecule by constructing a chart that shows how many ATP are produced at each stage of cellular respiration (both by substrate level phosphorylation and oxidative phsphorylation). Describe the fate of pyruvate in the absence of oxygen. Explain why fermentation is necessary. Distinguish between aerobic and anaerobic metabolism. Describe how food molecules other than glucose can be oxidized to make ATP and state the type of food molecule that produces the most ATP per gram. Explain 3 reasons scientists believe that glycolysis is considered one of the first metabolic pathways to have evolved. Explain what role the allosteric enzyme phosphofructokinase plays in the control of ATP production in the cell. AP Bio Ch 6 Goals ContinuedCellular Respiration

3. Cell Respiration ch 6 Break down of food molecules to release the energy stored in them to make ATP for the cells use. Type of rx? Delta G? Delta S? Oxidation – Reduction – Oxidizing agent – Reducing agent –

4. Energy flow through ecosystem

5. Overview of Cell Respiration http://media.pearsoncmg.com/bc/bc_camp bell_biology_7/media/interactivemedia/acti vities/load.html?9&Bhttp://media.pearsoncmg.com/bc/bc_camp bell_biology_7/media/interactivemedia/acti vities/load.html?9&B

6. Summary of Cell Res – 3 main parts

7. Just a reminder…

8. Summary of Glycolysis

9. Glycolysis breaks glucose into 2 molecules of pyruvate (which goes onto the next part of cell res) produces net gain of 2 ATP by sustrate level phosphorylation Produces 2 NADH occurs in cytoplasm no O needed

10. Glycolysis Energy investment phase Energy pay off phase

11. Animation of glycolysis http://henge.bio.miami.edu/mallery/movies/glycolysis. movhttp://henge.bio.miami.edu/mallery/movies/glycolysis. mov

12. Dehydrogenase – enzyme that removes H+ from glucose & intermediates of glucose & transfers it to NAD+ or FAD

13. Mitochondrial Structure

14. Oxidation of pyruvate – occurs after glycolysis & before Krebs as pyruvate enters mitochondrion CO 2 released e- transferred to 2NAD+, producing NADH Coenzyme A added to produce Acetyl CoA which enters Krebs cycle From this point out everything we go over occurs twice b/c in glycolysis glucose is broken into 2 molecules of pyruvate.

15. Krebs Cycle (aka Citric acid cycle) most enzymes for these rxns are located in mitochondrialmatrix 8 steps w/ 8 dif. enzymes oxaloacetate is recycled -2 Cs go in, 2 Cs come out in form of CO2 -cycle turns twice for every glu molecule oxidized

16. Krebs Cycle (aka Citric Acid Cycle)

17. Coenzymes involved in cell respiration are electron carriers – they transport the high E electrons from glucose & intermediates of glucose to the electron transport chain (ETC). NADH : has enough stored E to produce 3 ATPs through oxidative phosphorylation. FADH 2 : has enough stored E to produce 2 ATPs through oxidative phosphorylation

18. Electron Transport Chain (ETC) – transfers the E stored in NADH & FADH 2 to ATP

19. ETC Composed of proteins embedded in cristea – each one more electroneg. Than the previous. e- move from one to another. As each protein has the e-, it uses the E from it to pump H+ into the inner membrane space – creating an electrochemical gradient H+ rush through ATP synthase, the E released from rush is used to bond P to ADP making ATP - Chemiosmosis O is final e- acceptor & 2H+ are added to it to make H 2 O –breath out. NAD + & FAD can now go back to other steps of Cell Res to pick up more high E e-. http://www.youtube.com/watch?v=Id y2XAlZIVA

20. Chemiosmosis – using a proton gradient to make ATP Used in both cell res & photosynth esis to create ATP

21. Summary of Cell Res

22. Adding up the ATPs in made in Cell Res

23. What if O 2 isn’t present? Anaerobic respiration takes place Glycolysis + extra step to regenerate NAD+ so glycolysis can continue 2 types: Lactic Acid Ferm Alcoholic Ferm Pyruvate is final electron acceptor – creating lactic acid (aka lactate)

24. Alcoholic Fermentation

25. Cell Respiration & Fermentation Compared:

27. Control of Cellular Respiration Phosphofructokinase – an allosteric enzyme in the 3 rd step of glycolysis. –Acitvated by ADP –Inhibited by ATP & citrate Example of feedback inhibition.

28. STUDY!