1. Cell Respiration Chapter 5

2. Cellular Respiration Release of energy in biomolecules (food) and use of that energy to generate ATP ENERGY (food) + ADP + P i  ATP Two methods of breaking down food –Aerobic Respiration: oxygen utilizing –Anaerobic Respiration: no oxygen used

3. Aerobic Respiration Uses oxygen in breakdown of materials and release of energy –C 6 H 12 O 6 (glucose) + 6O 2  6CO 2 + 6H 2 O + Energy Energy is released in small increments via long metabolic pathways –Allows cells to efficiently release and use energy contained in food molecules

4. Aerobic Respiration: Overview Glycolysis –glucose  pyruvate Krebs Cycle –formation of electron carriers and CO 2 Oxidative Phosphorylation –electron carriers used to generate ATP

5. Glycolysis Occurs in cytoplasm of the cell Breakdown of one glucose molecule into two pyruvate molecules Yields 2 ATP molecules (net) and 2 NADH molecules

6. Glycolysis Glucose (6C) 2ATP 2ADP 2ADP 2ATP NAD + NADH Pyruvate (3C) NADH = high-energy electron carrier

7. Glycolysis Glucose  pyruvate 2 NAD + + 2H + + 4e -  2 NADH 2 ADP + 2 P i  2 ATP

8. Kreb’s Cycle Occurs in the mitochondrial matrix Cyclical series of reactions

9. Krebs Cycle: Acetyl-CoA Formation Pyruvate transported into mitochondrial matrix CO 2 cleaved off of pyruvate, forming acetate Acetate linked to Coenzyme A (CoA) to form acetyl-CoA One NADH formed for each pyruvate

10. Krebs Cycle (The Cycle Itself) Acetyl-CoA (2C) linked to oxaloacetic acid (4C), to form citric acid (6C) Citric acid ultimately converted into oxaloacetic acid + 2CO 2 1 GTP, 3 NADH and 1 FADH per each acetyl- CoA

11. Krebs Cycle Pyruvate (3C) NAD + NADH Citrate (6C) CO 2 Acetyl-CoA (2C) Oxaloacetate (4C) CO 2 3NAD + 3NADH FAD + FADH 2 GDP GTP FADH 2 – high energy electron carrier GTP – similar to ATP, can be converted to ATP

12. Krebs Cycle 3 CO 2, 1 GTP, 4 NADH and 1 FADH 2 produced for each pyruvate molecule. Total: 6CO 2, 2 GTP, 8 NADH, 2FADH 2

13. Oxidative Phosphorylation Occurs across the inner mitochondrial membrane Electrons from NADH and FADH 2 are transported along an electron transport chain Energy released used to produce ATP

14. Oxidative Phosphorylation H + pumped from inside the membrane to the outside –forms [H + ] gradient (more outside than inside) H + flows back in through ATP synthase –generates ATP Electrons and H + received by O 2 –forms H 2 O

15. Overall Reaction for Aerobic Respiration C 6 H 12 O 6 + 6O 2  6CO 2 + 6H 2 O

16. How Much ATP is Produced Per Glucose Molecule? Glycolysis 2 ATP Krebs Cycle(2 GTP) 2 ATP Oxidative Phosphorylation26 ATP TOTAL30 ATP

17. Triglyceride Catabolism Fatty acids are converted into acetyl-CoA Large amounts of ATP produced per fatty acid

18. Amino Acid Catabolism Different amino acids can be converted into various Krebs Cycle intermediates

19. Anaerobic Respiration Produce ATP in the absence of O 2 Used regularly by skeletal muscle fibers and RBCs Two steps: –Glycolysis - produce ATP –Lactate Formation – regenerate NAD +

20. Glycolysis in Anaerobiosis Glucose  Pyruvate Net 2 ATP produced NAD +  NADH Need NAD + to drive glycolysis!

21. Lactate Formation Pyruvate  Lactate NADH  NAD + Glycolysis can continue

22. How Much ATP is Produced Per Glucose Molecule? Glycolysis 2 ATP TOTAL 2 ATP Most of the energy from glucose is still present in the lactate Lactate accumulation leads to  pH